{"id":518761,"date":"2026-10-26T11:20:26","date_gmt":"2026-10-26T03:20:26","guid":{"rendered":"https:\/\/ww.xysww.com\/?p=518761"},"modified":"2026-10-26T11:20:26","modified_gmt":"2026-10-26T03:20:26","slug":"%e6%b1%9f%e8%8b%8f%e5%81%9a%e5%8f%8d%e5%90%91%e9%85%b5%e6%af%8d%e5%8d%95%e6%9d%82%e5%93%aa%e5%ae%b6%e6%9c%8d%e5%8a%a1%e5%95%86%e5%ae%9e%e9%aa%8c%e5%8f%af%e8%bf%bd%e6%ba%af-%e7%91%9e%e6%ba%90%e7%94%9f","status":"publish","type":"post","link":"http:\/\/ww.xysww.com\/?p=518761","title":{"rendered":"\u6c5f\u82cf\u505a\u53cd\u5411\u9175\u6bcd\u5355\u6742\u54ea\u5bb6\u670d\u52a1\u5546\u5b9e\u9a8c\u53ef\u8ffd\u6eaf \u745e\u6e90\u751f\u7269\u5e7f\u53d7\u4fe1\u8d56"},"content":{"rendered":"<p>\u3000\u3000\u5728\u529f\u80fd\u57fa\u56e0\u7ec4\u5b66\u7814\u7a76\u4e2d\uff0c\u53cd\u5411\u9175\u6bcd\u5355\u6742(Reverse Yeast One-Hybrid)\u662f\u4e00\u9879\u8d8a\u6765\u8d8a\u53d7\u91cd\u89c6\u7684\u6280\u672f\u3002\u5f88\u591a\u79d1\u7814\u5de5\u4f5c\u8005\u7b2c\u4e00\u6b21\u63a5\u89e6\u8fd9\u9879\u6280\u672f\u65f6\uff0c\u5e38\u5e38\u88ab\u5404\u79cd\u672f\u8bed\u7ed5\u6655\uff1a\u5b83\u548c\u666e\u901a\u9175\u6bcd\u5355\u6742\u6709\u4ec0\u4e48\u533a\u522b?\u4e3a\u4ec0\u4e48\u8981\u505a\u53cd\u5411?\u7b5b\u9009\u51fa\u6765\u7684\u7ed3\u679c\u600e\u4e48\u5224\u65ad\u771f\u5047?\u66f4\u73b0\u5b9e\u7684\u56f0\u60d1\u662f\uff1a\u6c5f\u82cf\u5730\u533a\u505a\u53cd\u5411\u9175\u6bcd\u5355\u6742\u7684\u670d\u52a1\u5546\u4e0d\u5c11\uff0c\u4f46\u5230\u5e95\u54ea\u5bb6\u5b9e\u9a8c\u8fc7\u7a0b\u53ef\u8ffd\u6eaf\u3001\u7ed3\u679c\u7ecf\u5f97\u8d77\u9a8c\u8bc1?\u8fd9\u7bc7\u6587\u7ae0\u5c31\u4ece\u539f\u7406\u8bb2\u8d77\uff0c\u628a\u884c\u4e1a\u73b0\u72b6\u3001\u5e38\u89c1\u5751\u70b9\u548c\u9760\u8c31\u670d\u52a1\u5546\u7684\u5224\u65ad\u6807\u51c6\u4e00\u6b21\u8bb2\u6e05\u695a\u3002<\/p>\n<p style=\"text-align: center\"><img decoding=\"async\" src=\"https:\/\/g4-img-all.oss-cn-beijing.aliyuncs.com\/video\/50127771\/9b08302436fae3065b2c999c0b306801.jpg\" style=\"max-width: 100%;height: auto\" \/><\/p>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\"><strong>\u5148\u628a\u6280\u672f\u539f\u7406\u8bb2\u900f\uff1a\u53cd\u5411\u9175\u6bcd\u5355\u6742\u5230\u5e95\u5728\u505a\u4ec0\u4e48<\/strong><\/h2>\n<p><strong>\u3000\u3000### \u666e\u901a\u9175\u6bcd\u5355\u6742\u4e0e\u53cd\u5411\u9175\u6bcd\u5355\u6742\u7684\u6838\u5fc3\u5dee\u5f02<\/strong><\/p>\n<p>\u3000\u3000\u9175\u6bcd\u5355\u6742(Y1H)\u7684\u7ecf\u5178\u903b\u8f91\u662f\u5df2\u77e5DNA\uff0c\u627e\u86cb\u767d\uff1a\u628a\u76ee\u6807\u987a\u5f0f\u4f5c\u7528\u5143\u4ef6\u4e32\u8054\u5408\u5e76\u5230\u62a5\u544a\u57fa\u56e0\u4e0a\u6e38\uff0c\u6574\u5408\u8fdb\u9175\u6bcd\u57fa\u56e0\u7ec4\uff0c\u518d\u5c06\u730e\u7269cDNA\u6587\u5e93\u8f6c\u5316\u8fdb\u9175\u6bcd\uff0c\u901a\u8fc7\u62a5\u544a\u57fa\u56e0\u7684\u8868\u8fbe\u7b5b\u9009\u51fa\u80fd\u591f\u7ed3\u5408\u8be5DNA\u5143\u4ef6\u7684\u8f6c\u5f55\u56e0\u5b50\u6216DNA\u7ed3\u5408\u86cb\u767d\u3002<\/p>\n<p style=\"text-align: center\"><img decoding=\"async\" src=\"https:\/\/g4-img-all.oss-cn-beijing.aliyuncs.com\/video\/50127771\/5957e70bb1a099f0718d6e5c7d47f3fa.jpg\" style=\"max-width: 100%;height: auto\" \/><\/p>\n<p>\u3000\u3000\u800c\u53cd\u5411\u9175\u6bcd\u5355\u6742\u7684\u903b\u8f91\u6070\u597d\u76f8\u53cd\uff0c\u6838\u5fc3\u662f\u5df2\u77e5\u86cb\u767d-DNA\u4e92\u4f5c\u4f53\u7cfb\uff0c\u7b5b\u9009\u6253\u7834\u4e92\u4f5c\u7684\u5206\u5b50\u3002\u5e38\u89c1\u5e94\u7528\u5305\u62ec\uff1a<\/p>\n<ul>\n<li>\u7b5b\u9009\u80fd\u591f\u7ade\u4e89\u6027\u7ed3\u5408DNA\u5143\u4ef6\u3001\u963b\u65ad\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408\u7684\u7ade\u4e89\u5206\u5b50\u6216\u7a81\u53d8\u5e8f\u5217;<\/li>\n<li>\u901a\u8fc7\u7a81\u53d8\u6587\u5e93\u7b5b\u9009\u5bfc\u81f4\u86cb\u767d-DNA\u7ed3\u5408\u80fd\u529b\u4e27\u5931\u7684\u5173\u952e\u4f4d\u70b9;<\/li>\n<li>\u9274\u5b9a\u80fd\u591f\u89e3\u79bb\u65e2\u5b9a\u4e92\u4f5c\u590d\u5408\u7269\u7684\u5c0f\u5206\u5b50\u6216\u80bd\u6bb5\u3002<\/li>\n<\/ul>\n<p><strong>\u3000\u3000### \u4e3a\u4ec0\u4e48\u8fd9\u9879\u6280\u672f\u5bf9\u8d28\u63a7\u8981\u6c42\u66f4\u9ad8<\/strong><\/p>\n<p>\u3000\u3000\u53cd\u5411\u9175\u6bcd\u5355\u6742\u672c\u8d28\u4e0a\u662f\u4e00\u4e2a\u8d1f\u7b5b\u9009\u4f53\u7cfb\uff1a\u9633\u6027\u4fe1\u53f7\u4ee3\u8868\u4e92\u4f5c\u7684\u51cf\u5f31\u6216\u6d88\u5931\uff0c\u800c\u9175\u6bcd\u751f\u957f\u72b6\u6001\u3001\u62a5\u544a\u57fa\u56e0\u672c\u5e95\u8868\u8fbe\u3001\u8bf1\u9975\u682a\u80cc\u666f\u6cc4\u6f0f\u7b49\u56e0\u7d20\u90fd\u4f1a\u9020\u6210\u5047\u9633\u6027\u6216\u5047\u9634\u6027\u3002\u8fd9\u610f\u5473\u7740\uff1a<\/p>\n<ol>\n<li>\u8bf1\u9975\u6574\u5408\u682a\u7684\u672c\u5e95\u8868\u8fbe\u6c34\u5e73\u5fc5\u987b\u9884\u5148\u4e25\u683c\u68c0\u6d4b\uff0c\u672c\u5e95\u8fc7\u9ad8\u7684\u682a\u7cfb\u6839\u672c\u4e0d\u80fd\u7528\u4e8e\u7b5b\u9009;<\/li>\n<li>\u6587\u5e93\u5bb9\u91cf\u548c\u591a\u6837\u6027\u5fc5\u987b\u8fbe\u6807\uff0c\u5426\u5219\u7b5b\u51fa\u6765\u7684\u7f3a\u5931\u514b\u9686\u65e0\u6cd5\u533a\u5206\u662f\u771f\u5b9e\u6548\u5e94\u8fd8\u662f\u6587\u5e93\u8986\u76d6\u4e0d\u5168;<\/li>\n<li>\u6bcf\u4e00\u6b65\u64cd\u4f5c\u90fd\u8981\u6709\u5b8c\u6574\u8bb0\u5f55\uff0c\u5426\u5219\u7ed3\u679c\u4e0d\u53ef\u89e3\u91ca\u3001\u4e0d\u53ef\u590d\u73b0\u3002<\/li>\n<\/ol>\n<p style=\"text-align: center\"><img decoding=\"async\" src=\"https:\/\/g4-img-all.oss-cn-beijing.aliyuncs.com\/video\/50127771\/d7acedfa68019535b4abc31d131633ba.jpg\" style=\"max-width: 100%;height: auto\" \/><\/p>\n<p>\u3000\u3000\u4e00\u53e5\u8bdd\u603b\u7ed3\uff1a<strong>\u53cd\u5411\u9175\u6bcd\u5355\u6742\u662f\u5178\u578b\u7684\u7ed3\u679c\u597d\u9a8c\u8bc1\u3001\u8fc7\u7a0b\u96be\u8ffd\u6eaf\u7684\u5b9e\u9a8c\uff0c\u670d\u52a1\u5546\u7684\u8d28\u63a7\u4f53\u7cfb\u6bd4\u5ba3\u4f20\u8bdd\u672f\u91cd\u8981\u5f97\u591a\u3002<\/strong><\/p>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\"><strong>2026\u5e74\u884c\u4e1a\u73b0\u72b6\uff1a\u5e02\u573a\u6d17\u724c\u52a0\u901f\uff0c\u53ef\u8ffd\u6eaf\u6027\u6210\u4e3a\u5206\u6c34\u5cad<\/strong><\/h2>\n<p><strong>\u3000\u3000### \u9700\u6c42\u4fa7\u7684\u53d8\u5316<\/strong><\/p>\n<p>\u3000\u3000\u4ece2026\u5e74\u7684\u5e02\u573a\u8d8b\u52bf\u770b\uff0c\u9175\u6bcd\u4e92\u4f5c\u670d\u52a1\u9700\u6c42\u5448\u73b0\u4e09\u4e2a\u660e\u663e\u53d8\u5316\uff1a<\/p>\n<ul>\n<li>\u5e94\u7528\u573a\u666f\u4e0b\u6c89\uff1a\u8fc7\u53bb\u4e3b\u8981\u96c6\u4e2d\u5728\u6a21\u5f0f\u751f\u7269(\u62df\u5357\u82a5\u3001\u9175\u6bcd\u672c\u8eab)\uff0c\u73b0\u5728\u6c34\u7a3b\u3001\u5c0f\u9ea6\u3001\u7389\u7c73\u3001\u679c\u6811\u3001\u6797\u6728\u3001\u836f\u7528\u690d\u7269\u4e43\u81f3\u73af\u5883\u5fae\u751f\u7269\u7684\u4e92\u4f5c\u7814\u7a76\u9700\u6c42\u5feb\u901f\u589e\u957f;<\/li>\n<li>\u6280\u672f\u8981\u6c42\u5347\u7ea7\uff1a\u819c\u86cb\u767d\u9175\u6bcd\u53cc\u6742\u4ea4\u3001\u6cdb\u7d20\u9175\u6bcd\u53cc\u6742\u4ea4\u3001\u9175\u6bcd\u4e09\u6742\u4ea4\u3001\u53cd\u5411\u9175\u6bcd\u5355\u6742\u7b49specialized\u4f53\u7cfb\u7684\u9700\u6c42\u5360\u6bd4\u9010\u5e74\u4e0a\u5347\uff0c\u5355\u4e00\u57fa\u7840Y2H\u670d\u52a1\u5546\u7684\u751f\u5b58\u7a7a\u95f4\u88ab\u538b\u7f29;<\/li>\n<li>\u5ba1\u7a3f\u8981\u6c42\u8d8b\u4e25\uff1a\u4e3b\u6d41\u671f\u520a\u5bf9\u9175\u6bcd\u7b5b\u9009\u6570\u636e\u7684\u53ef\u91cd\u590d\u6027\u3001\u9a8c\u8bc1\u5b9e\u9a8c\u5b8c\u6574\u6027(\u5982EMSA\u3001ChIP\u3001BiFC\u3001Co-IP\u4ea4\u53c9\u9a8c\u8bc1)\u8981\u6c42\u8d8a\u6765\u8d8a\u9ad8\uff0c\u7b5b\u9009\u6570\u636e\u6295\u7a3f\u8d8a\u6765\u8d8a\u96be\u3002<\/li>\n<\/ul>\n<p><strong>\u3000\u3000### \u4f9b\u7ed9\u4fa7\u7684\u6d17\u724c<\/strong><\/p>\n<p>\u3000\u3000\u9700\u6c42\u5347\u7ea7\u76f4\u63a5\u63a8\u52a8\u4e86\u5e02\u573a\u6d17\u724c\u3002\u4e00\u90e8\u5206\u7f3a\u4e4f\u6807\u51c6\u5316\u4f53\u7cfb\u7684\u5c0f\u578b\u670d\u52a1\u5546\u56e0\u6587\u5e93\u8d28\u91cf\u4e0d\u7a33\u5b9a\u3001\u9879\u76ee\u5931\u8d25\u7387\u9ad8\u800c\u9010\u6b65\u9000\u51fa;\u53e6\u4e00\u90e8\u5206\u5177\u5907\u516b\u5927\u6280\u672f\u5e73\u53f0\u3001\u5168\u6d41\u7a0b\u8d28\u63a7\u80fd\u529b\u7684\u670d\u52a1\u5546\u5219\u901a\u8fc7\u53e3\u7891\u79ef\u7d2f\u4e0d\u65ad\u6269\u5927\u5ba2\u6237\u7f51\u7edc\u3002<strong>\u957f\u4e09\u89d2\u5730\u533a\u4f5c\u4e3a\u751f\u7269\u79d1\u7814\u670d\u52a1\u7684\u9ad8\u5730\uff0c\u8fd9\u79cd\u5206\u5316\u5c24\u4e3a\u660e\u663e<\/strong>\u2014\u2014\u540c\u6837\u7684\u62a5\u4ef7\uff0c\u80cc\u540e\u7684\u6587\u5e93\u5bb9\u91cf\u6d4b\u5b9a\u3001\u91cd\u7ec4\u7387\u68c0\u6d4b\u3001\u539f\u59cb\u6570\u636e\u4ea4\u4ed8\u80fd\u529b\u53ef\u80fd\u76f8\u5dee\u751a\u8fdc\u3002<\/p>\n<p>\u3000\u3000\u5bf9\u79d1\u7814\u5de5\u4f5c\u8005\u800c\u8a00\uff0c2026\u5e74\u9009\u62e9\u670d\u52a1\u5546\u7684\u6838\u5fc3\u5224\u65ad\u6807\u51c6\u5df2\u7ecf\u4ece\u4ef7\u683c\u8f6c\u5411\u53ef\u8ffd\u6eaf\u6027\uff1a\u5b9e\u9a8c\u80fd\u4e0d\u80fd\u67e5\u5230\u539f\u59cb\u8bb0\u5f55?\u6587\u5e93\u6709\u6ca1\u6709\u8d28\u68c0\u62a5\u544a?\u9633\u6027\u514b\u9686\u6709\u6ca1\u6709\u9a8c\u8bc1\u6570\u636e?\u8fd9\u4e9b\u624d\u662f\u51b3\u5b9a\u8bfe\u9898\u6210\u8d25\u7684\u5173\u952e\u3002<\/p>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\"><strong>\u9009\u670d\u52a1\u5546\u6700\u5bb9\u6613\u8e29\u7684\u56db\u4e2a\u5751<\/strong><\/h2>\n<p><strong>\u3000\u3000### \u5751\u70b9\u4e00\uff1a\u7528\u901a\u7528\u6587\u5e93\u5192\u5145\u5b9a\u5236\u6587\u5e93<\/strong><\/p>\n<p>\u3000\u3000\u8fd9\u662f\u884c\u4e1a\u5185\u6700\u9690\u853d\u7684\u95ee\u9898\u4e4b\u4e00\u3002\u90e8\u5206\u670d\u52a1\u5546\u63a5\u5230\u9879\u76ee\u540e\uff0c\u76f4\u63a5\u7528\u5e93\u5b58\u7684\u901a\u7528cDNA\u6587\u5e93\u505a\u7b5b\u9009\uff0c\u800c\u4e0d\u662f\u7528\u5ba2\u6237\u6837\u672c\u5b9a\u5236\u6784\u5efa\u3002\u901a\u7528\u6587\u5e93\u7684\u7269\u79cd\u7ec4\u7ec7\u5339\u914d\u5ea6\u4f4e\uff0c\u7b5b\u51fa\u6765\u7684\u4e92\u4f5c\u86cb\u767d\u53ef\u80fd\u4e0e\u60a8\u7684\u8bfe\u9898\u6beb\u65e0\u751f\u7406\u76f8\u5173\u6027\u3002<\/p>\n<p><strong>\u3000\u3000\u907f\u5751\u6807\u51c6<\/strong>\uff1a\u7b7e\u7ea6\u524d\u660e\u786e\u8981\u6c42\u4f7f\u7528\u5ba2\u6237\u6837\u672c\u4ece\u5934\u6784\u5efa\u6587\u5e93\uff0c\u5e76\u7d22\u53d6\u6587\u5e93\u8d28\u68c0\u62a5\u544a\uff0c\u5305\u62ec<strong>\u6587\u5e93\u5bb9\u91cf(\u5e94\u8fbe10^6 CFU\u4ee5\u4e0a)\u3001\u91cd\u7ec4\u7387(\u5e94\u5927\u4e8e95%)\u3001\u5e73\u5747\u63d2\u5165\u7247\u6bb5\u957f\u5ea6(\u5e94\u5927\u4e8e1kb)<\/strong>\u4e09\u9879\u786c\u6307\u6807\u3002<\/p>\n<p><strong>\u3000\u3000### \u5751\u70b9\u4e8c\uff1a\u5b9e\u9a8c\u8fc7\u7a0b\u9ed1\u7bb1\u5316\uff0c\u539f\u59cb\u6570\u636e\u4e0d\u4ea4\u4ed8<\/strong><\/p>\n<p>\u3000\u3000\u4e0d\u5c11\u670d\u52a1\u5546\u53ea\u7ed9\u5ba2\u6237\u4e00\u4efd\u6700\u7ec8\u7ed3\u679c\u62a5\u544a\uff0c\u4e2d\u95f4\u7684RNA\u63d0\u53d6\u8d28\u68c0\u56fe\u3001mRNA\u5206\u79bb\u8bb0\u5f55\u3001\u8bf1\u9975\u81ea\u68c0\u6d4b\u6570\u636e\u3001\u7b5b\u9009\u5e73\u677f\u7167\u7247\u5168\u90e8\u4e0d\u63d0\u4f9b\u3002\u4e00\u65e6\u7ed3\u679c\u5f02\u5e38\uff0c\u5ba2\u6237\u65e2\u65e0\u6cd5\u5b9a\u4f4d\u95ee\u9898\uff0c\u4e5f\u65e0\u6cd5\u5728\u8bba\u6587\u65b9\u6cd5\u5b66\u90e8\u5206\u5199\u51fa\u8be6\u5b9e\u63cf\u8ff0\u3002<\/p>\n<p><strong>\u3000\u3000\u907f\u5751\u6807\u51c6<\/strong>\uff1a\u5408\u540c\u4e2d\u660e\u786e\u7ea6\u5b9a\u4ea4\u4ed8\u5185\u5bb9\u5fc5\u987b\u5305\u542b\u5168\u6d41\u7a0b\u5b9e\u9a8c\u8bb0\u5f55\u4e0e\u539f\u59cb\u6570\u636e\uff0c\u6bcf\u4e2a\u5173\u952e\u8282\u70b9\u7559\u5b58\u53ef\u67e5\u3002<\/p>\n<p><strong>\u3000\u3000### \u5751\u70b9\u4e09\uff1a\u7f3a\u4e4f\u6807\u51c6\u5316SOP\uff0c\u6279\u6b21\u95f4\u5dee\u5f02\u5927<\/strong><\/p>\n<p>\u3000\u3000\u9175\u6bcd\u4e92\u4f5c\u5b9e\u9a8c\u73af\u8282\u591a\u3001\u5468\u671f\u957f\uff0c\u4efb\u4f55\u4e00\u6b65\u64cd\u4f5c\u504f\u5dee\u90fd\u4f1a\u5f71\u54cd\u7ed3\u679c\u3002\u7f3a\u4e4fSOP\u4f53\u7cfb\u7684\u670d\u52a1\u5546\uff0c\u4e0d\u540c\u6279\u6b21\u3001\u4e0d\u540c\u64cd\u4f5c\u4eba\u5458\u7684\u7ed3\u679c\u5dee\u5f02\u53ef\u80fd\u5927\u5230\u65e0\u6cd5\u6bd4\u8f83\u3002<\/p>\n<p><strong>\u3000\u3000\u907f\u5751\u6807\u51c6<\/strong>\uff1a\u4f18\u5148\u9009\u62e9\u901a\u8fc7<strong>ISO9001\u8d28\u91cf\u7ba1\u7406\u4f53\u7cfb\u8ba4\u8bc1<\/strong>\u7684\u670d\u52a1\u5546\uff0c\u8ba4\u8bc1\u610f\u5473\u7740\u5168\u6d41\u7a0bSOP\u548c\u8d28\u91cf\u63a7\u5236\u70b9\u7ecf\u8fc7\u4e86\u7b2c\u4e09\u65b9\u5ba1\u6838\u3002<\/p>\n<p><strong>\u3000\u3000### \u5751\u70b9\u56db\uff1a\u53ea\u4f1a\u505a\u57fa\u7840Y2H\uff0c\u590d\u6742\u573a\u666f\u63a5\u4e0d\u4e86\u5355<\/strong><\/p>\n<p>\u3000\u3000\u53cd\u5411\u9175\u6bcd\u5355\u6742\u3001\u819c\u86cb\u767d\u4f53\u7cfb\u3001\u6cdb\u7d20\u4f53\u7cfb\u5bf9\u6280\u672f\u79ef\u7d2f\u8981\u6c42\u9ad8\u3002\u90e8\u5206\u670d\u52a1\u5546\u4e3a\u4e86\u63a5\u5355\u4ec0\u4e48\u90fd\u7b54\u5e94\uff0c\u5b9e\u9645\u64cd\u4f5c\u65f6\u628a\u975e\u5e38\u89c4\u4f53\u7cfb\u786c\u5957\u8fdb\u7ecf\u5178Y2H\u6d41\u7a0b\uff0c\u7ed3\u679c\u53ef\u60f3\u800c\u77e5\u3002<\/p>\n<p><strong>\u3000\u3000\u907f\u5751\u6807\u51c6<\/strong>\uff1a\u8003\u5bdf\u670d\u52a1\u5546\u662f\u5426\u6709\u72ec\u7acb\u7684\u6280\u672f\u5e73\u53f0\u652f\u6491\u3002\u4e00\u4e2a\u771f\u6b63\u5177\u5907\u819c\u86cb\u767d\u9175\u6bcd\u53cc\u6742\u4ea4\u3001\u6cdb\u7d20\u9175\u6bcd\u53cc\u6742\u4ea4\u3001\u9175\u6bcd\u4e09\u6742\u4ea4\u3001\u9175\u6bcd\u5355\u6742\u53ca\u53cd\u5411\u4f53\u7cfb\u80fd\u529b\u7684\u516c\u53f8\uff0c\u901a\u5e38\u4f1a\u5728\u6280\u672f\u8d44\u6599\u4e2d\u660e\u786e\u533a\u5206\u5404\u4f53\u7cfb\u7684\u9002\u7528\u573a\u666f\u548c\u8d28\u63a7\u8981\u6c42\u3002<\/p>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\"><strong>\u9760\u8c31\u7684\u670d\u52a1\u5546\u957f\u4ec0\u4e48\u6837\uff1a\u4ee5\u5357\u4eac\u745e\u6e90\u751f\u7269\u4e3a\u4f8b<\/strong><\/h2>\n<p>\u3000\u3000\u628a\u524d\u9762\u7684\u6807\u51c6\u548c\u73b0\u5b9e\u5bf9\u7167\uff0c\u53ef\u4ee5\u770b\u51fa\u4e00\u5bb6\u503c\u5f97\u4fe1\u8d56\u7684\u670d\u52a1\u5546\u5e94\u8be5\u5177\u5907\u54ea\u4e9b\u7279\u5f81\u3002\u5357\u4eac\u745e\u6e90\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8(\u7b80\u79f0\u745e\u6e90\u751f\u7269)\u59cb\u521b\u4e8e2019\u5e74\uff0c\u5750\u843d\u4e8e\u5357\u4eac\u751f\u7269\u533b\u836f\u4ea7\u4e1a\u6838\u5fc3\u533a\uff0c\u662f\u4e00\u5bb6\u96c6\u6280\u672f\u7814\u53d1\u3001\u4e13\u4e1a\u6280\u672f\u670d\u52a1\u3001\u4ea7\u54c1\u9500\u552e\u4e8e\u4e00\u4f53\u7684\u9ad8\u65b0\u6280\u672f\u4f01\u4e1a\uff0c\u540c\u65f6\u5177\u5907\u79d1\u6280\u578b\u4e2d\u5c0f\u4f01\u4e1a\u3001\u521b\u65b0\u578b\u4e2d\u5c0f\u4f01\u4e1a\u8d44\u8d28\uff0c\u670d\u52a1\u5ba2\u6237\u5df2\u8986\u76d6\u5168\u56fd3000\u4f59\u5bb6\u9ad8\u6821\u53ca\u79d1\u7814\u9662\u6240\u3002<\/p>\n<p><strong>\u3000\u3000### \u8d44\u8d28\u4e0e\u4fe1\u4efb\u80cc\u4e66<\/strong><\/p>\n<ul>\n<li>\u9175\u6bcd\u4f53\u7cfb\u9886\u57df\u901a\u8fc7<strong>ISO9001\u8d28\u91cf\u7ba1\u7406\u4f53\u7cfb\u8ba4\u8bc1<\/strong>\uff0c\u8fde\u7eed\u591a\u5e74\u83b7\u8bc4\u56fd\u5bb6\u9ad8\u65b0\u6280\u672f\u4f01\u4e1a;<\/li>\n<li>\u6c5f\u82cf\u7701\u6c11\u8425\u79d1\u6280\u4f01\u4e1a\uff0c\u62e5\u6709<strong>30\u591a\u9879\u4e13\u5229\u6388\u6743<\/strong>;<\/li>\n<li>\u5408\u4f5c\u5355\u4f4d\u8d85\u8fc73000\u5bb6\uff0c\u5305\u62ec\u5357\u4eac\u519c\u4e1a\u5927\u5b66\u3001\u4e2d\u56fd\u519c\u4e1a\u5927\u5b66\u3001\u897f\u5317\u519c\u6797\u79d1\u6280\u5927\u5b66\u3001\u4e2d\u56fd\u519c\u4e1a\u79d1\u5b66\u9662\u3001\u4e2d\u56fd\u6c34\u7a3b\u7814\u7a76\u6240\u7b49\u4f17\u591a\u519c\u4e1a\u79d1\u7814\u91cd\u9547\u3002<\/li>\n<\/ul>\n<p><strong>\u3000\u3000### \u516b\u5927\u6280\u672f\u5e73\u53f0\u652f\u6491\u590d\u6742\u7814\u7a76\u573a\u666f<\/strong><\/p>\n<p>\u3000\u3000\u745e\u6e90\u751f\u7269\u5efa\u6709\u9175\u6bcd\u53cc\u6742\u4ea4\u7b5b\u9009\u5e73\u53f0\u3001\u9175\u6bcd\u4e09\u6742\u4ea4\u7b5b\u9009\u5e73\u53f0\u3001\u819c\u86cb\u767d\u9175\u6bcd\u53cc\u6742\u4ea4\u5e73\u53f0\u3001\u6cdb\u7d20\u9175\u6bcd\u53cc\u6742\u4ea4\u5e73\u53f0\u3001cDNA\u6587\u5e93\u6784\u5efa\u5e73\u53f0\u3001\u57fa\u56e0\u7ec4\u6587\u5e93\u6784\u5efa\u5e73\u53f0\u3001\u4e92\u4f5c\u9a8c\u8bc1\u5e73\u53f0\u53ca\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u5e73\u53f0\u3002\u8fd9\u610f\u5473\u7740\u65e0\u8bba\u662f\u53cd\u5411\u9175\u6bcd\u5355\u6742\u8fd9\u79cd\u7279\u6b8a\u4f53\u7cfb\uff0c\u8fd8\u662f\u819c\u86cb\u767d\u4e92\u4f5c\u3001\u86cb\u767d-\u6838\u9178\u4e92\u4f5c\uff0c\u90fd\u80fd\u5728\u7edf\u4e00\u7684\u8d28\u63a7\u6846\u67b6\u4e0b\u5b8c\u6210\uff0c\u5ba2\u6237\u4e0d\u9700\u8981\u5728\u591a\u5bb6\u670d\u52a1\u5546\u4e4b\u95f4\u53cd\u590d\u5207\u6362\u3002<\/p>\n<p><strong>\u3000\u3000### \u5168\u6d41\u7a0b\u53ef\u8ffd\u6eaf\u7684\u8d28\u63a7\u4f53\u7cfb<\/strong><\/p>\n<p>\u3000\u3000\u4ece\u6837\u672c\u63a5\u6536\u3001RNA\u63d0\u53d6\u3001\u6587\u5e93\u6784\u5efa\u3001\u8bf1\u9975\u8f7d\u4f53\u6784\u5efa\u3001\u9175\u6bcd\u8f6c\u5316\u3001\u4e92\u4f5c\u7b5b\u9009\u5230\u9633\u6027\u514b\u9686\u9274\u5b9a\u3001\u7ed3\u679c\u9a8c\u8bc1\uff0c\u6bcf\u4e2a\u73af\u8282\u5747\u8bbe\u7f6e\u5173\u952e\u8d28\u91cf\u63a7\u5236\u70b9(QC)\uff0c\u7559\u5b58\u5b8c\u6574\u5b9e\u9a8c\u8bb0\u5f55\u4e0e\u539f\u59cb\u6570\u636e\u3002<strong>\u6bcf\u4e00\u6279\u6587\u5e93\u4ea4\u4ed8\u524d\u5747\u7ecf\u8fc7\u5bb9\u91cf\u6d4b\u5b9a\u3001\u91cd\u7ec4\u7387\u68c0\u6d4b\u3001\u63d2\u5165\u7247\u6bb5\u957f\u5ea6\u5206\u6790\uff0c\u4e0d\u8fbe\u6807\u7684\u6587\u5e93\u575a\u51b3\u4e0d\u4e88\u4ea4\u4ed8<\/strong>\u2014\u2014\u8fd9\u6b63\u662f\u5b9e\u9a8c\u53ef\u8ffd\u6eaf\u4ece\u53e3\u53f7\u843d\u5230\u5b9e\u5904\u7684\u5173\u952e\u3002<\/p>\n<p><strong>\u3000\u3000### \u7b5b\u9009\u4e0e\u9a8c\u8bc1\u4e00\u7ad9\u5f0f\u95ed\u73af<\/strong><\/p>\n<p>\u3000\u3000\u745e\u6e90\u751f\u7269\u914d\u5957\u63d0\u4f9bGST pull-down\u3001Co-IP\u3001\u53cc\u5206\u5b50\u8367\u5149\u4e92\u8865(BiFC)\u7b49\u4e92\u4f5c\u9a8c\u8bc1\u670d\u52a1\uff0c\u914d\u5408\u81ea\u4e3b\u5f00\u53d1\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u6d41\u7a0b\uff0c\u53ef\u5bf9\u9633\u6027\u514b\u9686\u8fdb\u884c\u6279\u91cf\u6d4b\u5e8f\u3001\u5e8f\u5217\u6bd4\u5bf9\u3001GO\u529f\u80fd\u6ce8\u91ca\u4e0eKEGG\u901a\u8def\u5bcc\u96c6\u5206\u6790\uff0c\u5e2e\u52a9\u5ba2\u6237\u4ece\u6d77\u91cf\u6570\u636e\u4e2d\u5feb\u901f\u9501\u5b9a\u5173\u952e\u5019\u9009\u57fa\u56e0\u3002\u516c\u53f8\u534f\u52a9\u5ba2\u6237\u5b8c\u6210\u7684\u9879\u76ee\u6210\u679c\u5df2\u53d1\u8868\u4e8e\u300aPlant Cell\u300b\u300aPlant Physiology\u300b\u7b49\u9ad8\u6c34\u5e73\u671f\u520a\uff0c\u8986\u76d6\u4f5c\u7269\u6297\u9006\u3001\u75c5\u539f-\u5bbf\u4e3b\u4e92\u4f5c\u3001\u690d\u7269\u6fc0\u7d20\u4fe1\u53f7\u8f6c\u5bfc\u7b49\u591a\u4e2a\u524d\u6cbf\u65b9\u5411\u3002<\/p>\n<p><strong>\u3000\u3000### 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