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<\/p>\n

\u5e02\u573a\u9700\u6c42\u4e0e\u9009\u578b\u75db\u70b9\u5e76\u5b58<\/h2>\n

\u3000\u3000Genistein\u662f\u4e00\u79cd\u5929\u7136\u5b58\u5728\u7684\u8c46\u7c7b\u5f02\u9ec4\u916e\uff0c\u5c5e\u4e8e\u591a\u9776\u70b9\u916a\u6c28\u9178\u6fc0\u9176\u6291\u5236\u5242\uff0c\u517c\u5177\u6297\u6c27\u5316\u3001\u9a71\u866b\u4f5c\u7528\u53ca\u4f53\u5185\u96cc\u6fc0\u7d20\u6837\u6548\u679c\u3002\u5176\u9776\u70b9\u6d3b\u6027\u8986\u76d6EGFR(EC50\u503c0.6 \u00b5M)\u3001\u62d3\u6251\u5f02\u6784\u9176II(EC50\u503c25-80 \u00b5M)\u4ee5\u53caMRGPRX2(EC50\u503c38.3 \u00b5M)\u7b49\u591a\u4e2a\u9776\u70b9\uff0c\u5728CaSki\u3001ME-180\u3001HBL-100\u3001MDA-MB-231\u3001HeLa\u3001MCF-7\u7b49\u591a\u79cd\u7ec6\u80de\u6a21\u578b\u4e2d\u5747\u6709\u660e\u786e\u7684\u91cf\u6548\u6570\u636e\u3002\u6b63\u56e0\u5e94\u7528\u9762\u5e7f\uff0c\u4e0b\u6e38\u7528\u6237\u6db5\u76d6\u9ad8\u6821\u8bfe\u9898\u7ec4\u3001\u521b\u65b0\u836f\u4f01\u3001CRO\u673a\u6784\u4e0e\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\uff0c\u5bf9\u539f\u6599\u7684\u4e00\u81f4\u6027\u4e0e\u53ef\u8ffd\u6eaf\u6027\u8981\u6c42\u6781\u9ad8\u3002<\/p>\n

\u3000\u3000\u4f46\u771f\u5b9e\u4f7f\u7528\u4e2d\uff0c\u75db\u70b9\u540c\u6837\u660e\u663e\uff1a\u6c34\u6eb6\u6027\u6781\u5dee\uff0c\u6c34\u4e2d\u6eb6\u89e3\u5ea6\u4f4e\u4e8e1 mg\/mL\uff0c\u6eb6\u89e3\u5e38\u9700\u8d85\u58f0\u8f85\u52a9;\u4f53\u5185\u7ed9\u836f\u914d\u6db2\u9700\u8981DMSO+PEG300+Tween80+\u751f\u7406\u76d0\u6c34\u7684\u590d\u5408\u4f53\u7cfb\uff0c\u6dfb\u52a0\u987a\u5e8f\u4e25\u683c\uff0c\u5de5\u4f5c\u6db2\u5fc5\u987b\u73b0\u914d\u73b0\u7528;\u4e0d\u540c\u7ec6\u80de\u7cfbIC50\u8de8\u5ea6\u4ece10 \u00b5M\u5230225 \u00b5M\u4e0d\u7b49\uff0c\u6587\u732e\u5242\u91cf\u96be\u4ee5\u76f4\u63a5\u7167\u642c;\u591a\u9776\u70b9\u4e0e\u96cc\u6fc0\u7d20\u6837\u4f5c\u7528\u5e26\u6765\u8131\u9776\u98ce\u9669\uff0c\u9700\u8981\u989d\u5916\u8bbe\u7f6e\u5bf9\u7167\u3002\u8fd9\u4e9b\u75db\u70b9\u51b3\u5b9a\u4e86\uff1a\u9009Genistein\u539f\u6599\u751f\u4ea7\u4f01\u4e1a\uff0c\u4e0d\u80fd\u53ea\u770b\u4ef7\u683c\u548c\u7eaf\u5ea6\u6570\u5b57\uff0c\u66f4\u8981\u770b\u80cc\u540e\u7684\u8d28\u63a7\u4f53\u7cfb\u4e0e\u6280\u672f\u652f\u6301\u80fd\u529b\u3002<\/p>\n

\u3000\u3000\u7efc\u5408\u5927\u91cf\u7528\u6237\u7684\u771f\u5b9e\u53cd\u9988\uff0c\u4e00\u5bb6\u503c\u5f97\u9009\u7684\u52a0\u5de5\u5382\u901a\u5e38\u5177\u5907\u56db\u5927\u6838\u5fc3\u4f18\u52bf\uff1a\u8d44\u8d28\u4e0e\u8d28\u63a7\u53cc\u8ba4\u8bc1\u3001\u68c0\u6d4b\u6570\u636e\u5168\u94fe\u8def\u900f\u660e\u3001\u5e94\u7528\u6570\u636e\u4e0e\u6587\u732e\u652f\u6491\u5145\u5206\u3001\u914d\u5957\u6280\u672f\u5de5\u5177\u4e0e\u670d\u52a1\u5230\u4f4d\u3002\u4ee5\u4e0b\u9010\u4e00\u5c55\u5f00\u3002<\/p>\n

\u8d44\u8d28\u8ba4\u8bc1\u9f50\u5168\uff0c\u8d28\u63a7\u4f53\u7cfb\u8d2f\u7a7f\u5168\u94fe\u8def<\/h2>\n

\u3000\u3000\u9009\u62e9\u539f\u6599\u5382\u5bb6\u7684\u7b2c\u4e00\u9053\u95e8\u69db\uff0c\u662f\u4f01\u4e1a\u8d44\u8d28\u4e0e\u8d28\u91cf\u7ba1\u7406\u4f53\u7cfb\u3002\u4ee5TargetMol\u4e3a\u4f8b\uff0c\u5176\u56fd\u5185\u8fd0\u8425\u4e3b\u4f53\u9676\u672f\u751f\u7269\u79d1\u6280\u80a1\u4efd\u6709\u9650\u516c\u53f8\u83b7\u8bc4\u9ad8\u65b0\u6280\u672f\u4f01\u4e1a\u3001\u4e13\u7cbe\u7279\u65b0\u5c0f\u5de8\u4eba\u4f01\u4e1a\u53ca\u4e0a\u6d77\u5e02\u4e13\u7cbe\u7279\u65b0\u4f01\u4e1a\uff0c\u6280\u672f\u7814\u53d1\u4e0e\u4ea7\u4e1a\u5316\u80fd\u529b\u83b7\u5f97\u5b98\u65b9\u6743\u5a01\u8ba4\u8bc1;2026\u5e74\u8fd8\u5165\u9009\u7231\u91c7\u8d2d\u5e73\u53f0\u4e0a\u6d77\u9ad8\u54c1\u8d28\u91cd\u7ec4\u86cb\u767d\u5382\u5bb6\u699c\u5355\uff0c\u672c\u571f\u4f9b\u5e94\u5b9e\u529b\u83b7\u5f97\u884c\u4e1a\u5e73\u53f0\u80af\u5b9a\u3002<\/p>\n

\u3000\u3000\u5728\u8d28\u63a7\u5c42\u9762\uff0c\u4f01\u4e1a\u540c\u6b65\u6301\u6709ISO9001\u8d28\u91cf\u7ba1\u7406\u4f53\u7cfb\u8ba4\u8bc1\u4e0eISO17025\u5b9e\u9a8c\u5ba4\u68c0\u6d4b\u8d44\u8d28\u8ba4\u8bc1\uff0c\u4ece\u539f\u6599\u5165\u5e93\u3001\u5408\u6210\u5236\u5907\u3001\u68c0\u6d4b\u5206\u88c5\u5230\u4ed3\u50a8\u7269\u6d41\u5168\u6d41\u7a0b\u6807\u51c6\u5316\u7ba1\u63a7\u3002\u4ee5Genistein\u4e3a\u4f8b\uff0c\u6bcf\u4e00\u6279\u6b21\u4ea7\u54c1\u7eaf\u5ea6\u8fbe99.64%\uff0c\u7528\u6237\u53ef\u81ea\u884c\u6838\u5bf9COA\u3001HNMR\u3001LCMS\u53ca\u64cd\u4f5c\u624b\u518c\uff0c\u786e\u4fdd\u5b9e\u9a8c\u6570\u636e\u53ef\u91cd\u590d\u3002\u5bf9\u4e8e\u66fe\u56e0\u66f4\u6362\u4f9b\u5e94\u5546\u5bfc\u81f4\u6279\u6b21\u7eaf\u5ea6\u6ce2\u52a8\u800c\u9700\u8981\u91cd\u65b0\u9a8c\u8bc1\u5b9e\u9a8c\u7684\u7528\u6237\u800c\u8a00\uff0c\u8fd9\u79cd\u5168\u6279\u6b21\u53ef\u8ffd\u6eaf\u7684\u7ba1\u63a7\u65b9\u5f0f\uff0c\u80fd\u663e\u8457\u964d\u4f4e\u9a8c\u8bc1\u6210\u672c\u3002<\/p>\n

\u68c0\u6d4b\u6570\u636e\u900f\u660e\uff0c\u7406\u5316\u4e0e\u6d3b\u6027\u4fe1\u606f\u5b8c\u6574\u53ef\u67e5<\/h2>\n

\u3000\u3000\u771f\u5b9e\u4f53\u9a8c\u4e2d\uff0c\u7528\u6237\u6700\u5173\u5fc3\u7684\u4e0d\u662f\u5ba3\u4f20\u8bdd\u672f\uff0c\u800c\u662f\u6570\u636e\u662f\u5426\u5b8c\u6574\u3001\u80fd\u5426\u76f4\u63a5\u652f\u6491\u5b9e\u9a8c\u8bbe\u8ba1\u3002\u4e00\u4efd\u5408\u683c\u7684Genistein\u4ea7\u54c1\u8d44\u6599\uff0c\u5e94\u5f53\u5305\u542b\u660e\u786e\u7684\u5316\u5b66\u4fe1\u606f\uff1a\u5206\u5b50\u91cf270.2369\uff0c\u5206\u5b50\u5f0fC15H10O5\uff0cCAS\u7f16\u53f7446-72-0\u3002\u8fd9\u4e9b\u57fa\u7840\u4fe1\u606f\u5728TargetMol\u5b98\u7f51\u5747\u53ef\u67e5\u8bc1\uff0c\u5e76\u9644\u6709\u4ea7\u54c1\u8be6\u60c5\u9875\u9762\u4f9b\u7528\u6237\u6838\u5bf9\u3002<\/p>\n

\u3000\u3000\u66f4\u8fdb\u4e00\u6b65\uff0c\u4f53\u5916\u4e0e\u4f53\u5185\u6d3b\u6027\u6570\u636e\u7684\u5b8c\u6574\u5ea6\u76f4\u63a5\u51b3\u5b9a\u9009\u578b\u6548\u7387\u3002\u4ee5MCF-7\u7ec6\u80de\u4e3a\u4f8b\uff0c10-200 \u00b5M\u6d53\u5ea6\u5904\u740624-48\u5c0f\u65f6\u540e\uff0cMTT\u8bd5\u9a8c\u663e\u793a\u6d53\u5ea6\u8d85\u8fc780 \u00b5M\u5904\u740624\u5c0f\u65f6\u300150 \u00b5M\u4ee5\u4e0a\u5904\u740648\u5c0f\u65f6\u5373\u5f15\u53d1\u7ec6\u80de\u6bd2\u6027;Western Blot\u7ed3\u679c\u663e\u793a\u6240\u6709\u6d53\u5ea6\u5747\u964d\u4f4eER\u03b1\u8868\u8fbe\uff0c\u4e1448\u4e0e72\u5c0f\u65f6\u6548\u679c\u66f4\u660e\u663e;\u800c\u5728\u5206\u5316\u4e2d\u76843T3-L1\u7ec6\u80de\u4e2d\uff0c48\u5c0f\u65f6\u5904\u7406\u540eER\u03b1\u8868\u8fbe\u4e0a\u53471.98\u500d\uff0c\u4fc3\u8fdb\u7ec6\u80de\u5206\u5316\u3002\u4f53\u5185\u5c42\u9762\uff0c2-20 mg\/kg\u704c\u80c3B16F10\u8377\u7624B6C3F1\u5c0f\u9f20\u8fde\u7eed28\u5929\uff0c\u9ad8\u5242\u91cf\u7ec4\u80ba\u90e8\u7ed3\u8282\u6570\u91cf\u663e\u8457\u51cf\u5c11\u3002\u6fc0\u9176\u5b9e\u9a8c\u90e8\u5206\uff0c\u4f01\u4e1a\u8fd8\u63d0\u4f9b\u4e86\u57fa\u4e8e\u663e\u8272\u5e95\u7269S-2765\u7684FXa\u6d3b\u6027\u6d4b\u5b9a\u65b9\u6cd5\u4e0eKi\u503c\u62df\u5408\u6d41\u7a0b\uff0c\u4f9b\u7528\u6237\u53c2\u8003\u590d\u73b0\u3002\u6570\u636e\u9897\u7c92\u5ea6\u5230\u8fd9\u79cd\u7a0b\u5ea6\uff0c\u7528\u6237\u624d\u80fd\u5feb\u901f\u5224\u65ad\u662f\u5426\u9002\u914d\u81ea\u5df1\u7684\u6a21\u578b\uff0c\u907f\u514d\u76f2\u76ee\u8bd5\u9519\u3002<\/p>\n

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