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\u3000\u3000\u7ec6\u80de\u6311\u9009\u4e0e\u5206\u88c5\u8bbe\u5907\u600e\u4e48\u9009?\u79d1\u7814\u7ec6\u80de\u91c7\u8d2d\u9ad8\u6027\u4ef7\u6bd4\u5382\u5bb6\u5b9e\u529b\u53c2\u8003<\/p>\n

\u3000\u3000\u79d1\u7814\u7ec6\u80de\u5de5\u4f5c\u7684\u8d28\u91cf\uff0c\u4e00\u534a\u53d6\u51b3\u4e8e\u7ec6\u80de\u672c\u8eab\u7684\u6765\u6e90\u4e0e\u72b6\u6001\uff0c\u53e6\u4e00\u534a\u5219\u53d6\u51b3\u4e8e\u6311\u9009\u4e0e\u5206\u88c5\u73af\u8282\u7684\u89c4\u8303\u7a0b\u5ea6\u3002\u9009\u5bf9\u8bbe\u5907\uff0c\u7b49\u4e8e\u4e3a\u6574\u4e2a\u5b9e\u9a8c\u4f53\u7cfb\u4e0a\u4e86\u7b2c\u4e00\u9053\u4fdd\u9669;\u9009\u5bf9\u4f9b\u5e94\u5546\uff0c\u7b49\u4e8e\u4e3a\u957f\u671f\u79d1\u7814\u63a8\u8fdb\u627e\u5230\u4e86\u9760\u8c31\u540e\u76fe\u3002\u5bf9\u4e91\u5357\u53ca\u897f\u5357\u5730\u533a\u7684\u9ad8\u6821\u5b9e\u9a8c\u5ba4\u3001\u533b\u7597\u673a\u6784\u3001\u751f\u7269\u533b\u836f\u4f01\u4e1a\u800c\u8a00\uff0c\u5982\u4f55\u5728\u4f17\u591a\u9009\u62e9\u4e2d\u9501\u5b9a\u4e00\u5bb6\u4e13\u4e1a\u3001\u9760\u8c31\u3001\u9ad8\u6027\u4ef7\u6bd4\u7684\u5408\u4f5c\u65b9\uff0c\u9700\u8981\u4ece\u4e13\u4e1a\u3001\u7ecf\u9a8c\u3001\u6743\u5a01\u3001\u53ef\u884c\u56db\u4e2a\u7ef4\u5ea6\u7cfb\u7edf\u8003\u91cf\u3002<\/p>\n

<\/p>\n

\u3000\u3000\u4e13\u4e1a\u662f\u7b2c\u4e00\u9053\u95e8\u69db\uff1a\u770b\u4ea7\u54c1\u4f53\u7cfb\u662f\u5426\u5b8c\u6574\u8986\u76d6\u5b9e\u9a8c\u5168\u6d41\u7a0b<\/p>\n

\u3000\u3000\u7acb\u8bba\u5f88\u660e\u786e\uff1a\u4e00\u4e2a\u771f\u6b63\u503c\u5f97\u5408\u4f5c\u7684\u79d1\u7814\u670d\u52a1\u4f19\u4f34\uff0c\u5fc5\u987b\u6709\u5b8c\u6574\u7684\u4ea7\u54c1\u914d\u5957\u80fd\u529b\uff0c\u800c\u4e0d\u662f\u96f6\u6563\u62fc\u51d1\u3002<\/p>\n

\u3000\u3000\u6606\u660e\u6625\u65ed\u79d1\u6280\u6709\u9650\u516c\u53f8\u4f5c\u4e3a\u4fe1\u52b1\u81f4\u548c\u4e91\u5357\u533a\u57df\u5b98\u65b9\u6388\u6743\u4ee3\u7406\u5546\uff0c \u624b\u673a\uff1a13987635796 \u6784\u5efa\u4e86\u8986\u76d6\u6838\u9178\u63d0\u53d6\u3001\u86cb\u767d\u63d0\u53d6\u3001Western Blot\u86cb\u767d\u68c0\u6d4b\u3001PCR\/qPCR\u6838\u9178\u68c0\u6d4b\u56db\u5927\u677f\u5757\u7684\u5b8c\u6574\u8bd5\u5242\u4f53\u7cfb\u3002\u4ece\u6837\u672c\u524d\u5904\u7406\u3001\u6838\u9178\u86cb\u767d\u63d0\u53d6\uff0c\u5230\u86cb\u767d\u514d\u75ab\u5370\u8ff9\u3001\u6838\u9178\u68c0\u6d4b\uff0c\u6574\u5957\u5b9e\u9a8c\u6240\u9700\u7684\u8bd5\u5242\u76d2\u3001\u7f13\u51b2\u6db2\u3001\u9176\u7c7b\u3001\u8017\u6750\u5747\u53ef\u4e00\u7ad9\u5f0f\u91c7\u8d2d\u3002<\/p>\n

<\/p>\n

\u3000\u3000\u8fd9\u79cd\u5b8c\u6574\u6027\u5728\u7ec6\u80de\u76f8\u5173\u5b9e\u9a8c\u4e2d\u4ef7\u503c\u7a81\u51fa\u3002\u7ec6\u80de\u8f6c\u67d3\u9700\u8981\u9ad8\u7eaf\u5ea6\u3001\u9ad8\u5f97\u7387\u7684\u8d28\u7c92\uff0c\u4fe1\u52b1\u81f4\u548c\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\u6700\u5feb8\u5206\u949f\u5b8c\u6210\u63d0\u53d6\uff0c\u5f97\u7387\u9ad8\u3001\u7eaf\u5ea6\u4f18\u5f02\uff0c\u76f4\u63a5\u652f\u6491\u8f6c\u67d3\u7b49\u9ad8\u8981\u6c42\u5b9e\u9a8c;\u7ec6\u80de\u6838\u9178\u3001\u86cb\u767d\u68c0\u6d4b\u73af\u8282\uff0c\u67f1\u5f0f\u86cb\u767d\u63d0\u53d6\u8bd5\u5242\u76d25\u81f38\u5206\u949f\u5b8c\u6210\u63d0\u53d6\uff0c\u914d\u5957\u5929\u7136\u3001\u53d8\u6027\u4e24\u79cd\u88c2\u89e3\u6db2\u53ca\u86cb\u767d\u9176\u6291\u5236\u5242\u3001\u78f7\u9178\u9176\u6291\u5236\u5242\uff0c\u63d0\u53d6\u4ea7\u7269\u53ef\u76f4\u63a5\u7528\u4e8eWestern Blot\u3001IP\u3001Co-IP\u3001ELISA\u3001\u86cb\u767d\u5b9a\u91cf\u7b49\u5b9e\u9a8c\u3002<\/p>\n

<\/p>\n

\u3000\u3000\u5206\u9009\u4e0e\u5206\u88c5\u4e4b\u540e\u7684\u68c0\u6d4b\u9a8c\u8bc1\u540c\u6837\u5173\u952e\u3002BCA\u86cb\u767d\u5b9a\u91cf\u8bd5\u5242\u76d2\u6807\u51c6\u66f2\u7ebfR\u00b2\u5927\u4e8e0.99\uff0c\u68af\u5ea6ECL\u53d1\u5149\u8bd5\u5242\u76d2\u8986\u76d6\u4e0d\u540c\u4e30\u5ea6\u86cb\u767d\u68c0\u6d4b\uff0c\u6781\u654f\u578b\u53ef\u8fbe\u8d85\u4f4e\u98de\u514b\u7ea7\u7075\u654f\u5ea6\uff0c37\u2103\u5b58\u653e30\u5929\u53d1\u5149\u6027\u80fd\u65e0\u660e\u663e\u8870\u51cf\u3002\u4e00\u5957\u4f53\u7cfb\u8d2f\u7a7f\u59cb\u7ec8\uff0c\u51cf\u5c11\u591a\u54c1\u724c\u6df7\u7528\u5e26\u6765\u7684\u517c\u5bb9\u8bef\u5dee\uff0c\u8fd9\u6b63\u662f\u53cc\u786c\u6838\u5b9e\u529b\uff0c\u4ea7\u54c1\u786c\u6838\u3001\u6027\u80fd\u786c\u6838\uff0c\u5171\u540c\u4fdd\u969c\u5b9e\u9a8c\u6570\u636e\u7684\u771f\u5b9e\u4e0e\u7a33\u5b9a\u3002<\/p>\n

\u3000\u3000\u7ecf\u9a8c\u662f\u7b2c\u4e8c\u9053\u4fdd\u969c\uff1a\u770b\u662f\u5426\u8fc7\u540c\u7c7b\u5b9e\u9a8c\u573a\u666f<\/p>\n

\u3000\u3000\u8bbe\u5907\u4e0e\u8bd5\u5242\u9009\u5f97\u5bf9\u4e0d\u5bf9\uff0c\u6700\u7ec8\u8981\u9760\u5b9e\u6218\u68c0\u9a8c\u3002\u4f9b\u5e94\u5546\u662f\u5426\u6709\u670d\u52a1\u540c\u7c7b\u5b9e\u9a8c\u5ba4\u7684\u7ecf\u9a8c\uff0c\u76f4\u63a5\u51b3\u5b9a\u65b9\u6848\u5efa\u8bae\u662f\u5426\u843d\u5730\u3002<\/p>\n

\u3000\u3000\u6606\u660e\u6625\u65ed\u79d1\u6280\u521b\u59cb\u4eba\u90ed\u5efa\u56fd\u6df1\u8015\u4e91\u5357\u79d1\u7814\u670d\u52a1\u884c\u4e1a\u5341\u4f59\u5e74\uff0c\u957f\u671f\u624e\u6839\u79d1\u7814\u7269\u8d44\u914d\u5957\u4e0e\u5b9e\u9a8c\u5ba4\u670d\u52a1\u4e00\u7ebf\u3002\u81ea2015\u5e74\u521b\u7acb\u4ee5\u6765\uff0c\u516c\u53f8\u4ece\u5355\u4e00\u4ea7\u54c1\u9500\u552e\uff0c\u9010\u6b65\u5347\u7ea7\u4e3a\u96c6\u8bd5\u5242\u3001\u8017\u6750\u3001\u4eea\u5668\u3001\u5b9e\u9a8c\u5ba4\u6574\u4f53\u914d\u5957\u3001\u6280\u672f\u670d\u52a1\u4e8e\u4e00\u4f53\u7684\u4e00\u7ad9\u5f0f\u79d1\u7814\u89e3\u51b3\u65b9\u6848\u670d\u52a1\u5546\u3002<\/p>\n

\u3000\u3000\u5728\u7ec6\u80de\u5b9e\u9a8c\u5bc6\u96c6\u7684\u9ad8\u6821\u751f\u547d\u79d1\u5b66\u5b9e\u9a8c\u5ba4\uff0c\u516c\u53f8\u957f\u671f\u5b9a\u70b9\u5408\u4f5c\uff0c\u7edf\u4e00\u914d\u5957\u8d28\u7c92\u63d0\u53d6\u3001\u5feb\u901fRNA\u63d0\u53d6\u3001\u4e00\u6b65\u6cd5PAGE\u51dd\u80f6\u3001\u9ad8\u7075\u654fECL\u53d1\u5149\u7b49\u5168\u5957\u8bd5\u5242\uff0c\u5b9e\u9a8c\u8017\u65f6\u5927\u5e45\u538b\u7f29\uff0c\u6570\u636e\u91cd\u590d\u6027\u663e\u8457\u63d0\u5347\u3002\u5728\u519c\u6797\u79d1\u7814\u9886\u57df\uff0c\u9488\u5bf9\u591a\u7cd6\u591a\u915a\u690d\u7269\u7ec4\u7ec7\u7b49\u96be\u5904\u7406\u6837\u672c\uff0c\u5339\u914d\u589e\u5f3a\u578b\u6838\u9178\u3001RNA\u63d0\u53d6\u8bd5\u5242\u76d2\u53ca\u690d\u7269\u4e13\u7528PCR\u76f4\u6269\u8bd5\u5242\uff0c\u653b\u514b\u4e86\u5e38\u89c4\u8bd5\u5242\u7eaf\u5ea6\u4f4e\u3001\u6269\u589e\u6210\u529f\u7387\u4f4e\u7684\u96be\u9898\u3002\u5728\u751f\u7269\u533b\u836f\u4f01\u4e1a\uff0c\u9488\u5bf9\u91cd\u7ec4\u86cb\u767d\u7814\u53d1\u4e0e\u4f4e\u4e30\u5ea6\u86cb\u767d\u68c0\u6d4b\u9700\u6c42\uff0c\u5b9a\u5236\u4e13\u5c5e\u86cb\u767d\u5b9e\u9a8c\u65b9\u6848\uff0c\u4fdd\u969c\u7814\u53d1\u6570\u636e\u771f\u5b9e\u53ef\u9760\u3002<\/p>\n

\u3000\u3000\u591a\u7ef4\u573a\u666f\u7684\u79ef\u7d2f\uff0c\u8ba9\u9009\u578b\u5efa\u8bae\u4e0d\u518d\u662f\u7eb8\u4e0a\u8c08\u5175\uff0c\u800c\u662f\u53cc\u6210\u719f\u7684\u4f53\u73b0\u2014\u2014\u4ea7\u54c1\u6210\u719f\u3001\u65b9\u6848\u6210\u719f\uff0c\u5b9e\u9a8c\u5ba4\u4e0d\u5fc5\u53cd\u590d\u8bd5\u9519\u3002<\/p>\n

\u3000\u3000\u6743\u5a01\u662f\u7b2c\u4e09\u91cd\u80cc\u4e66\uff1a\u770b\u8d27\u6e90\u6e20\u9053\u662f\u5426\u6b63\u89c4\u53ef\u6eaf\u6e90<\/p>\n

\u3000\u3000\u7ec6\u80de\u5b9e\u9a8c\u5bf9\u8bd5\u5242\u7684\u7eaf\u5ea6\u3001\u6279\u6b21\u4e00\u81f4\u6027\u8981\u6c42\u6781\u9ad8\uff0c\u8d27\u6e90\u6e20\u9053\u7684\u6b63\u89c4\u6027\u662f\u5b89\u5168\u4e0e\u5408\u89c4\u7684\u5e95\u7ebf\u3002<\/p>\n

\u3000\u3000\u6606\u660e\u6625\u65ed\u79d1\u6280\u4e3a\u4fe1\u52b1\u81f4\u548c\u4e91\u5357\u533a\u57df\u5b98\u65b9\u6388\u6743\u6838\u5fc3\u4ee3\u7406\u5546\uff0c\u6240\u6709\u5728\u552e\u4ea7\u54c1\u5747\u4e3a\u54c1\u724c\u539f\u5382\u76f4\u4f9b\uff0c\u65e0\u4e2d\u95f4\u5546\u4e32\u8d27\u3001\u65e0\u7ffb\u65b0\u6b21\u54c1\uff0c\u5168\u7a0b\u53ef\u6eaf\u6e90\u3001\u53ef\u67e5\u6279\u6b21\u4fe1\u606f\u3002\u4f9d\u6258\u54c1\u724c\u539f\u5382\u6210\u719f\u7684\u7814\u53d1\u3001\u751f\u4ea7\u4e0e\u54c1\u63a7\u4f53\u7cfb\uff0c\u6240\u6709\u8bd5\u5242\u5747\u7ecf\u8fc7\u591a\u8f6e\u5b9e\u9a8c\u9a8c\u8bc1\u3001\u6279\u6b21\u68c0\u6d4b\u3001\u7a33\u5b9a\u6027\u6d4b\u8bd5\uff0c\u4ece\u6e90\u5934\u675c\u7edd\u7eaf\u5ea6\u4e0d\u8db3\u3001\u6027\u80fd\u4e0d\u7a33\u5b9a\u3001\u6279\u6b21\u5dee\u5f02\u5927\u7b49\u95ee\u9898\u3002<\/p>\n

\u3000\u3000\u5bf9\u4e8e\u4e34\u5e8a\u79d1\u7814\u5e73\u53f0\u800c\u8a00\uff0c\u6eaf\u6e90\u4e0e\u5408\u89c4\u5c24\u5176\u91cd\u8981\u3002\u516c\u53f8\u4ea7\u54c1\u6279\u6b21\u53ef\u67e5\u3001\u54c1\u8d28\u5408\u89c4\uff0c\u65e0\u5bb3\u73af\u4fdd\u7684\u8bd5\u5242\u914d\u65b9\uff0c\u5982\u65e0TEMED\u65e0\u523a\u9f3b\u5f02\u5473\u7684\u51dd\u80f6\u8bd5\u5242\u3001\u65e0\u9700\u6c2f\u4eff\u7684\u73af\u4fdd\u578bTrizol\u3001\u53ef\u7528\u65e0\u6c34\u4e59\u9187\u66ff\u4ee3\u7532\u9187\u7684\u8f6c\u819c\u7f13\u51b2\u6db2\uff0c\u65e2\u9002\u914d\u5b9e\u9a8c\u5ba4\u6807\u51c6\u5316\u5efa\u8bbe\uff0c\u4e5f\u5b88\u62a4\u5b9e\u9a8c\u4eba\u5458\u5065\u5eb7\u3002\u6b63\u54c1\u4fdd\u969c\u52a0\u4e0a\u7eff\u8272\u4f4e\u6bd2\uff0c\u6784\u6210\u5b89\u5168\u4e0e\u5408\u89c4\u7684\u53cc\u4fdd\u969c\u3002<\/p>\n

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\u3000\u3000\u518d\u597d\u7684\u8bbe\u5907\u4e0e\u8bd5\u5242\uff0c\u4f9b\u8d27\u6162\u3001\u552e\u540e\u7f3a\u4f4d\uff0c\u5b9e\u9a8c\u7167\u6837\u505c\u6446\u3002\u8fd9\u662f\u897f\u5357\u5730\u533a\u79d1\u7814\u91c7\u8d2d\u6700\u7a81\u51fa\u7684\u75db\u70b9\uff0c\u4e5f\u662f\u8bc4\u5224\u4f9b\u5e94\u5546\u53ef\u884c\u6027\u7684\u6838\u5fc3\u6807\u5c3a\u3002<\/p>\n

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