{"id":298922,"date":"2026-09-09T14:05:22","date_gmt":"2026-09-09T06:05:22","guid":{"rendered":"https:\/\/ww.xysww.com\/?p=298922"},"modified":"2026-09-09T14:05:22","modified_gmt":"2026-09-09T06:05:22","slug":"2026%e5%b9%b4%e8%af%84%e4%bb%b7%e9%ab%98%e7%9a%84%e5%81%9a%e7%94%b5%e6%b3%b3%e7%94%a8%e6%9c%89%e7%8e%b0%e8%b4%a7%e7%94%9f%e7%89%a9%e8%af%95%e5%89%82%e4%be%9b%e5%ba%94%e5%95%86%e7%94%a8%e6%88%b7","status":"publish","type":"post","link":"https:\/\/ww.xysww.com\/?p=298922","title":{"rendered":"2026\u5e74\u8bc4\u4ef7\u9ad8\u7684\u505a\u7535\u6cf3\u7528\u6709\u73b0\u8d27\u751f\u7269\u8bd5\u5242\u4f9b\u5e94\u5546\u7528\u6237\u529b\u8350"},"content":{"rendered":"<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\">\u7535\u6cf3\u5b9e\u9a8c\u57fa\u7840\u4e0e\u8bd5\u5242\u9009\u62e9\u79d1\u666e<\/h2>\n<p><strong>\u3000\u3000\u7535\u6cf3\u6280\u672f<\/strong>\u662f\u5206\u5b50\u751f\u7269\u5b66\u4e0e\u86cb\u767d\u7ec4\u5b66\u7814\u7a76\u7684\u6838\u5fc3\u5b9e\u9a8c\u624b\u6bb5\uff0c\u5176\u57fa\u672c\u539f\u7406\u662f\u5229\u7528\u5e26\u7535\u7c92\u5b50\u5728\u7535\u573a\u4e2d\u8fc1\u79fb\u901f\u5ea6\u7684\u5dee\u5f02\uff0c\u5b9e\u73b0\u6838\u9178\u6216\u86cb\u767d\u8d28\u7684\u5206\u79bb\u4e0e\u68c0\u6d4b\u3002\u5728\u79d1\u7814\u5b9e\u9a8c\u5ba4\u4e2d\uff0c<strong>\u86cb\u767d\u514d\u75ab\u5370\u8ff9(Western Blot)<\/strong> \u548c<strong>\u6838\u9178\u7535\u6cf3<\/strong>\u662f\u6700\u5e38\u89c1\u7684\u4e24\u7c7b\u5e94\u7528\u573a\u666f\u3002<\/p>\n<p style=\"text-align: center\"><img decoding=\"async\" src=\"https:\/\/g4-img-all.oss-cn-beijing.aliyuncs.com\/video\/50124327\/276b9f726096adba6323994b278fdd1c.jpg\" style=\"max-width: 100%;height: auto\" 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Blot\u5b9e\u9a8c\u6d41\u7a0b<\/strong>\u901a\u5e38\u5305\u542b\u4ee5\u4e0b\u51e0\u4e2a\u5173\u952e\u6b65\u9aa4\uff1a<\/p>\n<ul>\n<li><strong>\u51dd\u80f6\u5236\u5907<\/strong>\uff1a\u6839\u636e\u76ee\u6807\u86cb\u767d\u5206\u5b50\u91cf\u5927\u5c0f\uff0c\u914d\u7f6e\u4e0d\u540c\u6d53\u5ea6\u7684\u5206\u79bb\u80f6\u4e0e\u6d53\u7f29\u80f6\uff0c\u4f20\u7edf\u65b9\u6cd5\u9700\u4f7f\u7528TEMED\u50ac\u5316\u805a\u5408\uff0c\u64cd\u4f5c\u7e41\u7410\u4e14\u6c14\u5473\u523a\u9f3b\u3002<\/li>\n<li><strong>\u7535\u6cf3\u5206\u79bb<\/strong>\uff1a\u5728\u6052\u5b9a\u7535\u538b\u6216\u7535\u6d41\u4e0b\uff0c\u86cb\u767d\u6837\u54c1\u7ecfSDS-PAGE\u51dd\u80f6\u5206\u79bb\uff0c\u5206\u5b50\u91cf\u5c0f\u7684\u86cb\u767d\u8fc1\u79fb\u901f\u5ea6\u5feb\uff0c\u5206\u5b50\u91cf\u5927\u7684\u86cb\u767d\u8fc1\u79fb\u901f\u5ea6\u6162\u3002<\/li>\n<li><strong>\u8f6c\u819c<\/strong>\uff1a\u5c06\u51dd\u80f6\u4e2d\u5206\u79bb\u7684\u86cb\u5e26\u8f6c\u79fb\u5230PVDF\u819c\u6216NC\u819c\u4e0a\uff0c\u4f20\u7edf\u65b9\u6cd5\u9700\u5728\u51b0\u6d74\u4e2d\u8fdb\u884c\uff0c\u8017\u65f6\u957f\u4e14\u4ea7\u70ed\u9ad8\u3002<\/li>\n<li><strong>\u5c01\u95ed\u4e0e\u6297\u4f53\u5b75\u80b2<\/strong>\uff1a\u4f7f\u7528\u5c01\u95ed\u6db2\u5c01\u95ed\u975e\u7279\u5f02\u6027\u7ed3\u5408\u4f4d\u70b9\uff0c\u4f9d\u6b21\u5b75\u80b2\u4e00\u6297\u548c\u4e8c\u6297\uff0c\u663e\u8272\u68c0\u6d4b\u76ee\u6807\u86cb\u767d\u4fe1\u53f7\u3002<\/li>\n<\/ul>\n<p style=\"text-align: center\"><img decoding=\"async\" src=\"https:\/\/g4-img-all.oss-cn-beijing.aliyuncs.com\/video\/50124327\/54e8afc05dbe7607b28153d59c6b0a50.jpg\" style=\"max-width: 100%;height: auto\" \/><\/p>\n<p><strong>\u3000\u3000\u6838\u9178\u7535\u6cf3<\/strong>\u5219\u4e3b\u8981\u7528\u4e8e\u9a8c\u8bc1PCR\u6269\u589e\u4ea7\u7269\u3001\u8d28\u7c92\u9176\u5207\u4ea7\u7269\u6216\u57fa\u56e0\u7ec4DNA\u5b8c\u6574\u6027\uff0c\u901a\u5e38\u4f7f\u7528\u743c\u8102\u7cd6\u51dd\u80f6\uff0c\u914d\u5408\u6838\u9178\u67d3\u6599\u5728\u7d2b\u5916\u706f\u4e0b\u89c2\u5bdf\u6761\u5e26\u3002<\/p>\n<p><strong>\u3000\u3000\u5f71\u54cd\u7535\u6cf3\u5b9e\u9a8c\u7ed3\u679c\u7684\u5173\u952e\u56e0\u7d20<\/strong>\u5305\u62ec\uff1a<\/p>\n<ul>\n<li><strong>\u51dd\u80f6\u8d28\u91cf<\/strong>\uff1a\u51dd\u80f6\u805a\u5408\u5747\u5300\u5ea6\u3001\u5b54\u5f84\u5927\u5c0f\u76f4\u63a5\u5f71\u54cd\u6761\u5e26\u5206\u79bb\u6548\u679c\u4e0e\u6e05\u6670\u5ea6\u3002<\/li>\n<li><strong>\u7f13\u51b2\u6db2\u4f53\u7cfb<\/strong>\uff1a\u7535\u6cf3\u7f13\u51b2\u6db2\u7684\u79bb\u5b50\u5f3a\u5ea6\u3001pH\u503c\u9700\u4fdd\u6301\u7a33\u5b9a\uff0c\u5426\u5219\u4f1a\u5bfc\u81f4\u6761\u5e26\u62d6\u5c3e\u6216\u8fc1\u79fb\u5f02\u5e38\u3002<\/li>\n<li><strong>\u6837\u54c1\u5904\u7406<\/strong>\uff1a\u86cb\u767d\u6837\u54c1\u9700\u5145\u5206\u53d8\u6027\u3001\u8fd8\u539f\uff0c\u6838\u9178\u6837\u54c1\u9700\u907f\u514d\u964d\u89e3\uff0c\u5426\u5219\u6761\u5e26\u6a21\u7cca\u6216\u51fa\u73b0\u975e\u7279\u5f02\u6027\u6761\u5e26\u3002<\/li>\n<li><strong>\u8f6c\u819c\u6548\u7387<\/strong>\uff1a\u8f6c\u819c\u65f6\u95f4\u3001\u7535\u538b\u3001\u7f13\u51b2\u6db2\u914d\u6bd4\u4e0d\u5f53\u4f1a\u5bfc\u81f4\u86cb\u767d\u8f6c\u79fb\u4e0d\u5b8c\u5168\uff0c\u5c24\u5176\u5c0f\u5206\u5b50\u86cb\u767d\u6613\u7a7f\u900f\u819c\u3002<\/li>\n<\/ul>\n<p style=\"text-align: center\"><img decoding=\"async\" src=\"https:\/\/g4-img-all.oss-cn-beijing.aliyuncs.com\/video\/50124327\/c13c29d7823748a1f2ae4e9353ab6ec6.jpg\" style=\"max-width: 100%;height: auto\" \/><\/p>\n<p><strong>\u3000\u3000\u9009\u62e9\u4f18\u8d28\u7535\u6cf3\u8bd5\u5242<\/strong>\u662f\u4fdd\u969c\u5b9e\u9a8c\u6210\u529f\u7684\u57fa\u7840\uff0c\u52a3\u8d28\u51dd\u80f6\u8bd5\u5242\u53ef\u80fd\u5bfc\u81f4\u805a\u5408\u4e0d\u5747\u3001\u6761\u5e26\u626d\u66f2;\u4f4e\u6548\u8f6c\u819c\u6db2\u4f1a\u5bfc\u81f4\u86cb\u767d\u4fe1\u53f7\u4e22\u5931;\u4e0d\u7a33\u5b9a\u7684\u663e\u8272\u6db2\u5219\u65e0\u6cd5\u51c6\u786e\u53cd\u6620\u76ee\u6807\u86cb\u767d\u4e30\u5ea6\u3002\u56e0\u6b64\uff0c\u79d1\u7814\u4eba\u5458\u9700\u8981\u4ece\u51dd\u80f6\u5236\u5907\u3001\u7535\u6cf3\u3001\u8f6c\u819c\u5230\u663e\u8272\u5168\u6d41\u7a0b\uff0c\u9009\u7528\u6027\u80fd\u7a33\u5b9a\u3001\u6279\u6b21\u4e00\u81f4\u3001\u64cd\u4f5c\u7b80\u4fbf\u7684\u8bd5\u5242\u4ea7\u54c1\uff0c\u624d\u80fd\u83b7\u5f97\u53ef\u9760\u3001\u53ef\u91cd\u590d\u7684\u5b9e\u9a8c\u6570\u636e\u3002<\/p>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\">\u7535\u6cf3\u8bd5\u5242\u884c\u4e1a\u5e02\u573a\u53d1\u5c55\u8d70\u5411\u5206\u6790<\/h2>\n<p>\u3000\u3000\u5f53\u524d\uff0c<strong>\u751f\u547d\u79d1\u5b66\u79d1\u7814\u8bd5\u5242\u5e02\u573a<\/strong>\u6b63\u7ecf\u5386\u4ece\u4f20\u7edf\u3001\u7e41\u7410\u64cd\u4f5c\u5411\u9ad8\u6548\u3001\u5b89\u5168\u3001\u73af\u4fdd\u65b9\u5411\u8f6c\u53d8\u7684\u6df1\u523b\u53d8\u9769\u3002\u5728\u7535\u6cf3\u76f8\u5173\u8bd5\u5242\u9886\u57df\uff0c\u4ee5\u4e0b\u8d8b\u52bf\u5c24\u4e3a\u660e\u663e\uff1a<\/p>\n<p><strong>\u3000\u30001. \u5b89\u5168\u73af\u4fdd\u6210\u4e3a\u6838\u5fc3\u8bc9\u6c42<\/strong><br \/>\n\u4f20\u7edf\u51dd\u80f6\u5236\u5907\u8bd5\u5242\u4e2d\uff0c<strong>TEMED<\/strong>\u5177\u6709\u5f3a\u70c8\u523a\u6fc0\u6027\u6c14\u5473\uff0c\u957f\u671f\u63a5\u89e6\u5bf9\u5b9e\u9a8c\u4eba\u5458\u547c\u5438\u9053\u548c\u76ae\u80a4\u9020\u6210\u4f24\u5bb3;\u8f6c\u819c\u7f13\u51b2\u6db2\u4e2d\u7684\u7532\u9187\u5c5e\u4e8e\u6709\u6bd2\u6709\u673a\u6eb6\u5242\uff0c\u9700\u8981\u4e25\u683c\u7ba1\u63a7\u3002\u5e02\u573a\u5bf9\u3001\u65e0\u5473\u3001\u4f4e\u5bb3\u8bd5\u5242\u7684\u9700\u6c42\u65e5\u76ca\u589e\u957f\u3002\u8d8a\u6765\u8d8a\u591a\u7684\u5b9e\u9a8c\u5ba4\u5f00\u59cb\u9009\u7528<strong>\u65e0TEMED\u4e00\u6b65\u6cd5PAGE\u51dd\u80f6\u8bd5\u5242\u76d2<\/strong>\uff0c\u8fd9\u7c7b\u4ea7\u54c1\u901a\u8fc7\u4f18\u5316\u914d\u65b9\uff0c\u65e0\u9700\u6dfb\u52a0\u523a\u6fc0\u6027\u6210\u5206\u5373\u53ef\u5feb\u901f\u805a\u5408\uff0c\u5927\u5e45\u6539\u5584\u5b9e\u9a8c\u73af\u5883\u3002<\/p>\n<p><strong>\u3000\u30002. \u5b9e\u9a8c\u6548\u7387\u4e0e\u4fbf\u6377\u6027\u4f18\u5148<\/strong><br \/>\n\u79d1\u7814\u8282\u594f\u52a0\u5feb\uff0c\u5b9e\u9a8c\u5ba4\u5bf9<strong>\u7f29\u77ed\u5b9e\u9a8c\u5468\u671f<\/strong>\u7684\u8981\u6c42\u8d8a\u6765\u8d8a\u9ad8\u3002\u4f20\u7edf\u51dd\u80f6\u5236\u5907\u970030\u5206\u949f\u4ee5\u4e0a\u7b49\u5f85\u51dd\u56fa\uff0c\u8f6c\u819c\u9700\u51b0\u6d74\u8017\u65f61\u5c0f\u65f6\u4ee5\u4e0a\u3002\u5e02\u9762\u4e0a\u6d8c\u73b0\u51fa<strong>\u5feb\u901f\u51dd\u80f6\u8bd5\u5242\u76d2<\/strong>\uff0c\u53ef\u572825\u5206\u949f\u5185\u5b8c\u6210\u7535\u6cf3;<strong>\u901f\u6eb6\u7f13\u51b2\u9897\u7c92<\/strong>\u52a0\u6c34\u5373\u7528\uff0c\u65e0\u9700\u79f0\u91cf\u914d\u5236;<strong>\u5373\u7528\u578b\u9884\u5236\u80f6<\/strong>\u5f00\u888b\u5373\u53ef\u4e0a\u6837\uff0c\u517c\u5bb9\u4e3b\u6d41\u7535\u6cf3\u69fd\u3002\u8fd9\u4e9b\u4ea7\u54c1\u5e2e\u52a9\u79d1\u7814\u4eba\u5458\u5c06\u66f4\u591a\u65f6\u95f4\u805a\u7126\u4e8e\u5b9e\u9a8c\u8bbe\u8ba1\u800c\u975e\u91cd\u590d\u6027\u64cd\u4f5c\u3002<\/p>\n<p><strong>\u3000\u30003. \u4ea7\u54c1\u7ebf\u4e00\u4f53\u5316\u4e0e\u517c\u5bb9\u6027\u589e\u5f3a<\/strong><br \/>\n\u4ee5\u5f80\uff0c\u5b9e\u9a8c\u5ba4\u9700\u8981\u4ece\u4e0d\u540c\u4f9b\u5e94\u5546\u91c7\u8d2d\u51dd\u80f6\u8bd5\u5242\u3001\u7535\u6cf3\u7f13\u51b2\u6db2\u3001\u8f6c\u819c\u6db2\u3001\u86cb\u767dMarker\u3001\u53d1\u5149\u6db2\u7b49\u4ea7\u54c1\uff0c\u4e0d\u540c\u54c1\u724c\u95f4\u7684\u517c\u5bb9\u6027\u96be\u4ee5\u4fdd\u8bc1\uff0c\u5b9e\u9a8c\u8bef\u5dee\u98ce\u9669\u9ad8\u3002\u5982\u4eca\uff0c<strong>\u4e00\u7ad9\u5f0f\u8bd5\u5242\u54c1\u724c<\/strong>\u63d0\u4f9b\u4ece\u51dd\u80f6\u5236\u5907\u5230\u663e\u5f71\u7684\u5168\u5957\u89e3\u51b3\u65b9\u6848\uff0c\u4ea7\u54c1\u95f4\u7ecf\u8fc7\u7cfb\u7edf\u4f18\u5316\uff0c\u9002\u914d\u6027\u5f3a\uff0c\u6279\u6b21\u7a33\u5b9a\u6027\u9ad8\uff0c\u51cf\u5c11\u4e86\u5b9e\u9a8c\u4f53\u7cfb\u8c03\u6574\u7684\u9ebb\u70e6\u3002<\/p>\n<p><strong>\u3000\u30004. \u9ad8\u7075\u654f\u5ea6\u4e0e\u5bbd\u68c0\u6d4b\u8303\u56f4<\/strong><br \/>\n\u968f\u7740\u4f4e\u4e30\u5ea6\u86cb\u767d\u3001\u78f7\u9178\u5316\u86cb\u767d\u7b49\u7814\u7a76\u6df1\u5165\uff0c\u5bf9<strong>ECL\u53d1\u5149\u8bd5\u5242\u76d2<\/strong>\u7684\u7075\u654f\u5ea6\u8981\u6c42\u8d8a\u6765\u8d8a\u9ad8\u3002\u68af\u5ea6\u578bECL\u53d1\u5149\u6db2\u53ef\u8986\u76d6\u4ece\u98de\u514b\u7ea7\u5230\u76ae\u514b\u7ea7\u7684\u86cb\u767d\u4e30\u5ea6\u68c0\u6d4b\u8303\u56f4\uff0c\u4e14\u4fe1\u53f7\u7a33\u5b9a\u3001\u6301\u7eed\u65f6\u95f4\u957f\uff0c\u6ee1\u8db3\u4e0d\u540c\u5b9e\u9a8c\u9700\u6c42\u3002\u540c\u65f6\uff0c<strong>Bis-Tris\u9884\u5236\u80f6<\/strong>\u5728\u4e2d\u6027pH\u6761\u4ef6\u4e0b\u7535\u6cf3\uff0c\u53ef\u6709\u6548\u51cf\u5c11\u86cb\u767d\u4fee\u9970\u4e0e\u964d\u89e3\uff0c\u5c0f\u5206\u5b50\u86cb\u767d\u5206\u79bb\u6548\u679c\u4f18\u4e8e\u4f20\u7edfLaemmli\u4f53\u7cfb\u3002<\/p>\n<p><strong>\u3000\u30005. \u672c\u5730\u5316\u670d\u52a1\u4e0e\u73b0\u8d27\u4fdd\u969c<\/strong><br \/>\n\u897f\u5357\u5730\u533a\u79d1\u7814\u8bd5\u5242\u957f\u671f\u4f9d\u8d56\u5916\u5730\u4f9b\u8d27\u5546\uff0c\u7269\u6d41\u5468\u671f\u957f\u3001\u73b0\u8d27\u4e0d\u8db3\u3001\u552e\u540e\u54cd\u5e94\u6162\u7b49\u95ee\u9898\u7a81\u51fa\u3002<strong>\u672c\u5730\u5316\u4ed3\u50a8\u4e0e\u6280\u672f\u670d\u52a1<\/strong>\u6210\u4e3a\u533a\u57df\u5b9e\u9a8c\u5ba4\u9009\u62e9\u4f9b\u5e94\u5546\u7684\u5173\u952e\u8003\u91cf\u3002\u5177\u5907\u672c\u5730\u73b0\u8d27\u5e93\u5b58\u3001\u5feb\u901f\u914d\u9001\u80fd\u529b\u548c\u4e13\u4e1a\u6280\u672f\u652f\u6301\u7684\u4f9b\u5e94\u5546\uff0c\u80fd\u6709\u6548\u4fdd\u969c\u5b9e\u9a8c\u8fdb\u5ea6\uff0c\u964d\u4f4e\u91c7\u8d2d\u98ce\u9669\u3002<\/p>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\">\u5ba2\u6237\u9009\u8d2d\u7535\u6cf3\u8bd5\u5242\u7684\u5e38\u89c1\u8e29\u5751\u8981\u70b9\u4e0e\u907f\u5751\u77e5\u8bc6<\/h2>\n<p>\u3000\u3000\u5728\u9009\u8d2d\u7535\u6cf3\u8bd5\u5242\u8fc7\u7a0b\u4e2d\uff0c\u79d1\u7814\u4eba\u5458\u5e38\u56e0\u4fe1\u606f\u4e0d\u5bf9\u79f0\u6216\u7ecf\u9a8c\u4e0d\u8db3\u800c\u8e29\u5751\uff0c\u5bfc\u81f4\u5b9e\u9a8c\u5931\u8d25\u6216\u6570\u636e\u5931\u771f\u3002\u4ee5\u4e0b\u603b\u7ed3<strong>\u516d\u5927\u5e38\u89c1\u8e29\u5751\u8981\u70b9<\/strong>\u53ca\u5bf9\u5e94\u7684<strong>\u907f\u5751\u7b56\u7565<\/strong>\uff1a<\/p>\n<p><strong>\u3000\u30001. \u51dd\u80f6\u805a\u5408\u4e0d\u5747\u6216\u6279\u6b21\u5dee\u5f02\u5927<\/strong><\/p>\n<ul>\n<li><strong>\u8e29\u5751\u8868\u73b0<\/strong>\uff1a\u540c\u4e00\u6279\u6b21\u51dd\u80f6\u51fa\u73b0\u90e8\u5206\u533a\u57df\u805a\u5408\u4e0d\u5b8c\u5168\uff0c\u6216\u4e0d\u540c\u6279\u6b21\u51dd\u80f6\u7684\u5206\u79bb\u6548\u679c\u5dee\u5f02\u660e\u663e\uff0c\u6761\u5e26\u8fc1\u79fb\u4f4d\u7f6e\u4e0d\u4e00\u81f4\u3002<\/li>\n<li><strong>\u907f\u5751\u77e5\u8bc6<\/strong>\uff1a\u9009\u7528<strong>\u77e5\u540d\u54c1\u724c\u6216\u7ecf\u8fc7\u9a8c\u8bc1\u7684\u51dd\u80f6\u8bd5\u5242\u76d2<\/strong>\uff0c\u4f18\u5148\u9009\u62e9<strong>\u5373\u7528\u578b\u9884\u5236\u80f6<\/strong>\u6216<strong>\u4e00\u6b65\u6cd5\u5feb\u901f\u51dd\u80f6\u8bd5\u5242\u76d2<\/strong>\uff0c\u8fd9\u7c7b\u4ea7\u54c1\u914d\u65b9\u6210\u719f\u3001\u805a\u5408\u6761\u4ef6\u4f18\u5316\uff0c\u6279\u6b21\u7a33\u5b9a\u6027\u9ad8\u3002\u8d2d\u4e70\u524d\u8981\u6c42\u4f9b\u5e94\u5546\u63d0\u4f9b\u6279\u6b21\u8d28\u68c0\u62a5\u544a\uff0c\u786e\u8ba4\u4ea7\u54c1\u5728\u591a\u4e2a\u5b9e\u9a8c\u5ba4\u7684\u9a8c\u8bc1\u6570\u636e\u3002<\/li>\n<\/ul>\n<p><strong>\u3000\u30002. \u8f6c\u819c\u6548\u7387\u4f4e\uff0c\u86cb\u767d\u4fe1\u53f7\u4e22\u5931<\/strong><\/p>\n<ul>\n<li><strong>\u8e29\u5751\u8868\u73b0<\/strong>\uff1a\u8f6c\u819c\u540e\u819c\u4e0a\u86cb\u5e26\u6a21\u7cca\u3001\u4fe1\u53f7\u5f31\uff0c\u5c24\u5176\u5c0f\u5206\u5b50\u86cb\u767d(&lt;20 kDa)\u5bb9\u6613\u7a7f\u900f\u819c\uff0c\u5927\u5206\u5b50\u86cb\u767d(&gt;150 kDa)\u8f6c\u79fb\u4e0d\u5b8c\u5168\u3002<\/li>\n<li><strong>\u907f\u5751\u77e5\u8bc6<\/strong>\uff1a\u9009\u62e9<strong>\u914d\u5957\u8f6c\u819c\u6db2\u4e0e\u8f6c\u819c\u6761\u4ef6<\/strong>\uff0c\u4f7f\u7528<strong>\u4f4e\u70ed\u91cf\u5feb\u901f\u8f6c\u819c\u6db2<\/strong>\u53ef\u907f\u514d\u51b0\u6d74\uff0c\u63d0\u9ad8\u8f6c\u819c\u6548\u7387\u3002\u8f6c\u819c\u524d\u786e\u8ba4PVDF\u819c\u6216NC\u819c\u5b54\u5f84\u4e0e\u76ee\u6807\u86cb\u767d\u5206\u5b50\u91cf\u5339\u914d\uff0c\u5c0f\u5206\u5b50\u86cb\u767d\u9009\u75280.2 \u03bcm\u5b54\u5f84\u819c\uff0c\u5927\u5206\u5b50\u86cb\u767d\u9009\u75280.45 \u03bcm\u5b54\u5f84\u819c\u3002\u8f6c\u819c\u65f6\u95f4\u9700\u6839\u636e\u86cb\u767d\u5927\u5c0f\u4f18\u5316\uff0c\u4e00\u822c\u5c0f\u5206\u5b50\u86cb\u767d\u8f6c\u819c15-20\u5206\u949f\uff0c\u5927\u5206\u5b50\u86cb\u767d\u970030-60\u5206\u949f\u3002<\/li>\n<\/ul>\n<p><strong>\u3000\u30003. ECL\u53d1\u5149\u6db2\u7075\u654f\u5ea6\u4e0d\u8db3\u6216\u4fe1\u53f7\u4e0d\u7a33\u5b9a<\/strong><\/p>\n<ul>\n<li><strong>\u8e29\u5751\u8868\u73b0<\/strong>\uff1a\u4f4e\u4e30\u5ea6\u86cb\u767d\u65e0\u6cd5\u68c0\u51fa\uff0c\u6216\u53d1\u5149\u4fe1\u53f7\u5feb\u901f\u8870\u51cf\uff0c\u96be\u4ee5\u83b7\u5f97\u6e05\u6670\u56fe\u50cf\u3002<\/li>\n<li><strong>\u907f\u5751\u77e5\u8bc6<\/strong>\uff1a\u6839\u636e\u76ee\u6807\u86cb\u767d\u4e30\u5ea6\u9009\u62e9<strong>\u68af\u5ea6\u578bECL\u53d1\u5149\u6db2<\/strong>\uff0c\u4f8b\u5982\u6781\u654f\u578b\u53ef\u8fbe\u98de\u514b\u7ea7\u7075\u654f\u5ea6\uff0c\u666e\u901a\u578b\u9002\u5408\u9ad8\u4e30\u5ea6\u86cb\u767d\u3002\u53d1\u5149\u6db2\u9700\u907f\u5149\u4fdd\u5b58\uff0c\u4f7f\u7528\u524d\u6062\u590d\u81f3\u5ba4\u6e29\u3002\u907f\u514d\u53d1\u5149\u6db2\u4e0e\u91d1\u5c5e\u79bb\u5b50\u63a5\u89e6\uff0c\u4f7f\u7528\u5e72\u51c0\u7684\u5bb9\u5668\u548c\u79fb\u6db2\u67aa\u5934\u3002\u663e\u5f71\u524d\u53ef\u5c06\u819c\u4e0e\u53d1\u5149\u6db2\u5b75\u80b22-5\u5206\u949f\uff0c\u786e\u4fdd\u4fe1\u53f7\u7a33\u5b9a\u3002<\/li>\n<\/ul>\n<p><strong>\u3000\u30004. \u7f13\u51b2\u6db2\u914d\u5236\u9519\u8bef\u5bfc\u81f4\u7535\u6cf3\u5f02\u5e38<\/strong><\/p>\n<ul>\n<li><strong>\u8e29\u5751\u8868\u73b0<\/strong>\uff1a\u7535\u6cf3\u65f6\u7535\u6d41\u5f02\u5e38\u3001\u6761\u5e26\u626d\u66f2\u3001\u62d6\u5c3e\uff0c\u6216Marker\u8fc1\u79fb\u4f4d\u7f6e\u4e0d\u51c6\u786e\u3002<\/li>\n<li><strong>\u907f\u5751\u77e5\u8bc6<\/strong>\uff1a\u4f7f\u7528<strong>\u7535\u6cf3\u901f\u6eb6\u7f13\u51b2\u9897\u7c92<\/strong>\uff0c\u76f4\u63a5\u52a0\u6c34\u6eb6\u89e3\u5373\u53ef\uff0c\u907f\u514d\u79f0\u91cf\u8bef\u5dee\u3002\u914d\u5236\u65f6\u4f7f\u7528\u53bb\u79bb\u5b50\u6c34\uff0c\u907f\u514d\u6742\u8d28\u79bb\u5b50\u5e72\u6270\u3002\u7535\u6cf3\u7f13\u51b2\u6db2\u9700\u73b0\u914d\u73b0\u7528\u62164\u5ea6\u4fdd\u5b58\uff0c\u4e0d\u5b9c\u53cd\u590d\u4f7f\u7528\u3002\u8f6c\u819c\u7f13\u51b2\u6db2\u9700\u6ce8\u610f\u7532\u9187\u6216\u4e59\u9187\u6d53\u5ea6\uff0c\u4f7f\u7528\u65e0\u6c34\u4e59\u9187\u66ff\u4ee3\u7532\u9187\u53ef\u964d\u4f4e\u6bd2\u6027\uff0c\u4f46\u9700\u786e\u8ba4\u914d\u65b9\u517c\u5bb9\u6027\u3002<\/li>\n<\/ul>\n<p><strong>\u3000\u30005. \u86cb\u767dMarker\u9009\u62e9\u4e0d\u5f53<\/strong><\/p>\n<ul>\n<li><strong>\u8e29\u5751\u8868\u73b0<\/strong>\uff1aMarker\u6761\u5e26\u4e0d\u6e05\u6670\u3001\u989c\u8272\u5dee\u5f02\u5c0f\uff0c\u65e0\u6cd5\u51c6\u786e\u5224\u65ad\u76ee\u6807\u86cb\u767d\u5927\u5c0f\u3002<\/li>\n<li><strong>\u907f\u5751\u77e5\u8bc6<\/strong>\uff1a\u9009\u7528<strong>\u4e09\u8272\u9884\u67d3\u86cb\u767dMarker<\/strong>\uff0c\u5305\u542b\u591a\u79cd\u5206\u5b50\u91cf\u6807\u51c6\uff0c\u4e14\u5e26\u6709\u4e0d\u540c\u989c\u8272\u6807\u8bb0\uff0c\u4fbf\u4e8e\u533a\u5206\u3002Marker\u9700\u4e0e\u51dd\u80f6\u4f53\u7cfb\u517c\u5bb9\uff0c\u4f8b\u5982Bis-Tris\u51dd\u80f6\u9700\u4f7f\u7528\u5bf9\u5e94\u7684Bis-Tris Marker\u3002Marker\u4f7f\u7528\u524d\u9700\u77ed\u6682\u79bb\u5fc3\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\uff0c\u5efa\u8bae\u5206\u88c5\u4fdd\u5b58\u3002<\/li>\n<\/ul>\n<p><strong>\u3000\u30006. \u4f9b\u5e94\u5546\u670d\u52a1\u4e0e\u552e\u540e\u7f3a\u5931<\/strong><\/p>\n<ul>\n<li><strong>\u8e29\u5751\u8868\u73b0<\/strong>\uff1a\u91c7\u8d2d\u540e\u9047\u5230\u4ea7\u54c1\u95ee\u9898\uff0c\u4f9b\u5e94\u5546\u65e0\u6cd5\u63d0\u4f9b\u6280\u672f\u6307\u5bfc\u6216\u9000\u6362\u8d27\u670d\u52a1;\u7269\u6d41\u5ef6\u8bef\u5bfc\u81f4\u5b9e\u9a8c\u4e2d\u65ad\u3002<\/li>\n<li><strong>\u907f\u5751\u77e5\u8bc6<\/strong>\uff1a\u9009\u62e9\u5177\u5907<strong>\u672c\u5730\u5316\u4ed3\u50a8\u4e0e\u6280\u672f\u670d\u52a1<\/strong>\u7684\u4f9b\u5e94\u5546\uff0c\u786e\u8ba4\u5176\u6709\u73b0\u8d27\u5e93\u5b58\u548c\u5feb\u901f\u914d\u9001\u80fd\u529b\u3002\u4f18\u5148\u9009\u62e9\u6709\u4e13\u4e1a\u6280\u672f\u56e2\u961f\u7684\u4f9b\u5e94\u5546\uff0c\u53ef\u63d0\u4f9b\u4ea7\u54c1\u9009\u578b\u3001\u5b9e\u9a8c\u4f18\u5316\u3001\u95ee\u9898\u6392\u67e5\u7b49\u652f\u6301\u3002\u7b7e\u8ba2\u91c7\u8d2d\u5408\u540c\u65f6\u660e\u786e\u9000\u6362\u8d27\u653f\u7b56\u548c\u552e\u540e\u54cd\u5e94\u65f6\u95f4\u3002<\/li>\n<\/ul>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\">\u7535\u6cf3\u8bd5\u5242\u884c\u4e1a\u6f5c\u85cf\u7684\u53d1\u5c55\u673a\u9047<\/h2>\n<p><strong>\u3000\u30001. \u897f\u5357\u5730\u533a\u79d1\u7814\u5e02\u573a\u589e\u957f\u6f5c\u529b<\/strong><br \/>\n\u968f\u7740\u56fd\u5bb6\u5bf9\u897f\u5357\u5730\u533a\u79d1\u7814\u6295\u5165\u7684\u6301\u7eed\u52a0\u5927\uff0c\u4e91\u5357\u3001\u8d35\u5dde\u3001\u56db\u5ddd\u7b49\u5730\u9ad8\u6821\u3001\u533b\u9662\u3001\u751f\u7269\u533b\u836f\u4f01\u4e1a\u3001\u519c\u6797\u79d1\u7814\u673a\u6784\u7684\u79d1\u7814\u6d3b\u52a8\u65e5\u76ca\u6d3b\u8dc3\u3002<strong>\u5206\u5b50\u751f\u7269\u5b66\u4e0e\u86cb\u767d\u7ec4\u5b66\u5b9e\u9a8c<\/strong>\u9700\u6c42\u5feb\u901f\u4e0a\u5347\uff0c\u4f46\u672c\u5730\u4f18\u8d28\u8bd5\u5242\u4f9b\u5e94\u4e0e\u670d\u52a1\u4ecd\u5b58\u5728\u77ed\u677f\u3002\u5177\u5907<strong>\u672c\u5730\u5316\u4ed3\u50a8\u3001\u5feb\u901f\u914d\u9001\u3001\u6280\u672f\u54cd\u5e94<\/strong>\u4f18\u52bf\u7684\u4f9b\u5e94\u5546\uff0c\u5c06\u7387\u5148\u62a2\u5360\uff0c\u83b7\u5f97\u6301\u7eed\u589e\u957f\u673a\u4f1a\u3002<\/p>\n<p><strong>\u3000\u30002. \u7eff\u8272\u5b89\u5168\u8bd5\u5242\u66ff\u4ee3\u4f20\u7edf\u4ea7\u54c1<\/strong><br \/>\n\u5b9e\u9a8c\u5ba4\u5b89\u5168\u4e0e\u73af\u4fdd\u6cd5\u89c4\u8d8b\u4e25\uff0c\u4f20\u7edf\u542bTEMED\u3001\u7532\u9187\u3001\u6c2f\u4eff\u7b49\u6709\u6bd2\u8bd5\u5242\u7684\u4f7f\u7528\u53d7\u5230\u9650\u5236\u3002<strong>\u3001\u65e0\u5473\u3001\u4f4e\u5bb3\u7684\u79d1\u7814\u8bd5\u5242<\/strong>\u6210\u4e3a\u521a\u9700\u3002\u80fd\u591f\u63d0\u4f9b<strong>\u65e0TEMED\u51dd\u80f6\u8bd5\u5242\u3001\u73af\u4fdd\u578b\u8f6c\u819c\u6db2\u3001\u6838\u9178\u63d0\u53d6\u8bd5\u5242<\/strong>\u7684\u4f9b\u5e94\u5546\uff0c\u5c06\u83b7\u5f97\u5b9e\u9a8c\u5ba4\u7684\u9752\u7750\uff0c\u5c24\u5176\u662f\u9ad8\u6821\u548c\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\uff0c\u5bf9\u5b89\u5168\u5408\u89c4\u8981\u6c42\u66f4\u9ad8\u3002<\/p>\n<p><strong>\u3000\u30003. \u4e00\u7ad9\u5f0f\u89e3\u51b3\u65b9\u6848\u9700\u6c42\u589e\u957f<\/strong><br \/>\n\u5b9e\u9a8c\u5ba4\u91c7\u8d2d\u6548\u7387\u63d0\u5347\u9700\u6c42\u63a8\u52a8<strong>\u4e00\u7ad9\u5f0f\u8bd5\u5242\u914d\u5957\u670d\u52a1<\/strong>\u53d1\u5c55\u3002\u4ece\u6838\u9178\u63d0\u53d6\u3001\u86cb\u767d\u63d0\u53d6\u5230Western Blot\u3001PCR\u68c0\u6d4b\uff0c\u5168\u6d41\u7a0b\u8bd5\u5242\u7edf\u4e00\u54c1\u724c\u3001\u7edf\u4e00\u91c7\u8d2d\uff0c\u53ef\u51cf\u5c11\u517c\u5bb9\u6027\u95ee\u9898\u548c\u91c7\u8d2d\u7ba1\u7406\u6210\u672c\u3002\u4f9b\u5e94\u5546\u82e5\u80fd\u63d0\u4f9b<strong>\u5168\u5b9e\u9a8c\u6d41\u7a0b\u7684\u4ea7\u54c1\u7ebf<\/strong>\uff0c\u5e76\u914d\u5957\u5b9e\u9a8c\u65b9\u6848\u4f18\u5316\u3001\u6280\u672f\u57f9\u8bad\u7b49\u589e\u503c\u670d\u52a1\uff0c\u5c06\u663e\u8457\u63d0\u5347\u5ba2\u6237\u7c98\u6027\u3002<\/p>\n<p><strong>\u3000\u30004. \u9ad8\u901a\u91cf\u5b9e\u9a8c\u4e0e\u81ea\u52a8\u5316\u9002\u914d<\/strong><br \/>\n\u836f\u7269\u7814\u53d1\u3001\u4e34\u5e8a\u68c0\u6d4b\u3001\u80b2\u79cd\u7b5b\u9009\u7b49\u9886\u57df\u5bf9<strong>\u9ad8\u901a\u91cf\u5b9e\u9a8c<\/strong>\u9700\u6c42\u65fa\u76db\u3002\u5373\u7528\u578b\u9884\u5236\u80f6\u3001\u9884\u6df7\u6db2\u3001\u901f\u6eb6\u7f13\u51b2\u9897\u7c92\u7b49\u4ea7\u54c1\uff0c\u53ef\u9002\u914d\u81ea\u52a8\u5316\u79fb\u6db2\u5de5\u4f5c\u7ad9\u3001\u9ad8\u901a\u91cf\u7535\u6cf3\u69fd\u7b49\u8bbe\u5907\uff0c\u51cf\u5c11\u4eba\u5de5\u64cd\u4f5c\u8bef\u5dee\uff0c\u63d0\u9ad8\u5b9e\u9a8c\u6548\u7387\u3002\u4f9b\u5e94\u5546\u9700\u8981\u5173\u6ce8<strong>\u81ea\u52a8\u5316\u5b9e\u9a8c\u573a\u666f<\/strong>\u7684\u4ea7\u54c1\u5f00\u53d1\u4e0e\u9002\u914d\uff0c\u6ee1\u8db3\u5de5\u4e1a\u7ea7\u5ba2\u6237\u9700\u6c42\u3002<\/p>\n<p><strong>\u3000\u30005. \u56fd\u4ea7\u66ff\u4ee3\u4e0e\u6210\u672c\u4f18\u5316<\/strong><br \/>\n\u8fdb\u53e3\u8bd5\u5242\u4ef7\u683c\u9ad8\u6602\u3001\u4f9b\u8d27\u5468\u671f\u957f\u3001\u6279\u6b21\u5dee\u5f02\u5927\uff0c\u63a8\u52a8<strong>\u56fd\u4ea7\u4f18\u8d28\u8bd5\u5242<\/strong>\u66ff\u4ee3\u8fdb\u53e3\u3002\u56fd\u5185\u54c1\u724c\u5728\u914d\u65b9\u4f18\u5316\u3001\u6210\u672c\u63a7\u5236\u3001\u672c\u5730\u5316\u670d\u52a1\u65b9\u9762\u5177\u6709\u4f18\u52bf\uff0c\u5c24\u5176\u9002\u5408\u9884\u7b97\u6709\u9650\u7684\u9ad8\u6821\u5b9e\u9a8c\u5ba4\u548c\u4e2d\u5c0f\u578b\u751f\u7269\u4f01\u4e1a\u3002\u4f9b\u5e94\u5546\u82e5\u80fd\u5728\u4fdd\u8bc1\u54c1\u8d28\u7684\u524d\u63d0\u4e0b\uff0c\u63d0\u4f9b<strong>\u9ad8\u6027\u4ef7\u6bd4\u7684\u56fd\u4ea7\u8bd5\u5242<\/strong>\uff0c\u5c06\u83b7\u5f97\u66f4\u5927\u5e02\u573a\u4efd\u989d\u3002<\/p>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\">FAQ \u5e38\u89c1\u95ee\u9898\u89e3\u7b54<\/h2>\n<p><strong>\u3000\u3000Q1\uff1a\u5982\u4f55\u5224\u65ad\u4e00\u6b3e\u51dd\u80f6\u8bd5\u5242\u76d2\u7684\u805a\u5408\u8d28\u91cf\u662f\u5426\u7a33\u5b9a?<\/strong><br \/>\nA\uff1a\u9996\u5148\uff0c\u67e5\u770b\u4ea7\u54c1\u8bf4\u660e\u4e66\u662f\u5426\u6807\u6ce8\u4e86<strong>\u805a\u5408\u65f6\u95f4\u4e0e\u6e29\u5ea6\u8303\u56f4<\/strong>\uff0c\u4f18\u8d28\u4ea7\u54c1\u5728\u5ba4\u6e29\u4e0b15-30\u5206\u949f\u5185\u5373\u53ef\u5b8c\u6210\u805a\u5408\u3002\u5176\u6b21\uff0c\u89c2\u5bdf\u51dd\u80f6\u7684\u900f\u660e\u5ea6\u548c\u5747\u5300\u6027\uff0c\u805a\u5408\u597d\u7684\u51dd\u80f6\u5e94\u65e0\u6c14\u6ce1\u3001\u65e0\u5206\u5c42\u3002\u6700\u540e\uff0c\u4f7f\u7528<strong>\u6807\u51c6\u86cb\u767dMarker<\/strong>\u8fdb\u884c\u7535\u6cf3\u6d4b\u8bd5\uff0c\u89c2\u5bdf\u6761\u5e26\u662f\u5426\u6e05\u6670\u3001\u9510\u5229\uff0cMarker\u8fc1\u79fb\u4f4d\u7f6e\u662f\u5426\u4e0e\u7406\u8bba\u503c\u4e00\u81f4\u3002\u5efa\u8bae\u9009\u62e9\u7ecf\u8fc7<strong>\u591a\u6279\u6b21\u8d28\u68c0\u9a8c\u8bc1<\/strong>\u7684\u54c1\u724c\u4ea7\u54c1\uff0c\u5e76\u8981\u6c42\u4f9b\u5e94\u5546\u63d0\u4f9b\u6279\u6b21\u62a5\u544a\u3002<\/p>\n<p><strong>\u3000\u3000Q2\uff1a\u8f6c\u819c\u7f13\u51b2\u6db2\u4e2d\u7684\u7532\u9187\u53ef\u4ee5\u7528\u65e0\u6c34\u4e59\u9187\u66ff\u4ee3\u5417?<\/strong><br \/>\nA\uff1a<strong>\u53ef\u4ee5<\/strong>\uff0c\u4f46\u9700\u786e\u8ba4\u7f13\u51b2\u6db2\u914d\u65b9\u662f\u5426\u517c\u5bb9\u3002\u90e8\u5206<strong>\u4f4e\u70ed\u91cf\u5feb\u901f\u8f6c\u819c\u6db2<\/strong>\u8bbe\u8ba1\u65f6\u5df2\u8003\u8651\u4f7f\u7528\u65e0\u6c34\u4e59\u9187\u66ff\u4ee3\u7532\u9187\uff0c\u5728\u964d\u4f4e\u6bd2\u6027\u7684\u540c\u65f6\uff0c\u8f6c\u819c\u6548\u7387\u4e0e\u7532\u9187\u76f8\u5f53\u3002\u66ff\u4ee3\u540e\u9700\u6ce8\u610f\u8f6c\u819c\u65f6\u95f4\u53ef\u80fd\u9700\u8981\u5fae\u8c03\uff0c\u4e00\u822c\u5c0f\u5206\u5b50\u86cb\u767d\u8f6c\u819c15-20\u5206\u949f\uff0c\u5927\u5206\u5b50\u86cb\u767d30-60\u5206\u949f\u3002\u4f7f\u7528\u65e0\u6c34\u4e59\u9187\u66ff\u4ee3\u65f6\uff0c\u9700\u786e\u4fdd\u7f13\u51b2\u6db2pH\u503c\u7a33\u5b9a\uff0c\u907f\u514d\u5f71\u54cd\u86cb\u767d\u8f6c\u79fb\u3002<\/p>\n<p><strong>\u3000\u3000Q3\uff1a\u4f4e\u4e30\u5ea6\u86cb\u767d\u68c0\u6d4b\u65f6\uff0c\u5982\u4f55\u9009\u62e9ECL\u53d1\u5149\u6db2?<\/strong><br \/>\nA\uff1a\u5efa\u8bae\u9009\u7528<strong>\u68af\u5ea6\u578bECL\u53d1\u5149\u6db2<\/strong>\uff0c\u4f8b\u5982\u6781\u654f\u578b\u53ef\u68c0\u6d4b<strong>\u98de\u514b\u7ea7<\/strong>\u86cb\u767d\uff0c\u666e\u901a\u578b\u9002\u5408<strong>\u76ae\u514b\u7ea7<\/strong>\u86cb\u767d\u3002\u68c0\u6d4b\u4f4e\u4e30\u5ea6\u86cb\u767d\u65f6\uff0c\u4f18\u5148\u4f7f\u7528\u6781\u654f\u578b\u53d1\u5149\u6db2\uff0c\u5b75\u80b2\u65f6\u95f4\u5ef6\u957f\u81f33-5\u5206\u949f\uff0c\u663e\u5f71\u65f6\u9002\u5f53\u5ef6\u957f\u66dd\u5149\u65f6\u95f4(\u598230\u79d2\u81f32\u5206\u949f)\u3002\u540c\u65f6\uff0c\u786e\u4fdd\u86cb\u767d\u4e0a\u6837\u91cf\u8db3\u591f(20-50 \u03bcg)\uff0c\u8f6c\u819c\u6548\u7387\u9ad8\uff0c\u907f\u514d\u86cb\u767d\u635f\u5931\u3002\u53d1\u5149\u6db2\u9700\u907f\u5149\u4fdd\u5b58\uff0c\u4f7f\u7528\u524d\u6062\u590d\u81f3\u5ba4\u6e29\uff0c\u907f\u514d\u4fe1\u53f7\u8870\u51cf\u3002<\/p>\n<p><strong>\u3000\u3000Q4\uff1a\u9884\u5236\u80f6\u4e0e\u81ea\u914d\u51dd\u80f6\u76f8\u6bd4\uff0c\u6709\u54ea\u4e9b\u4f18\u52bf?<\/strong><br \/>\nA\uff1a\u9884\u5236\u80f6\u7684\u4e3b\u8981\u4f18\u52bf\u5305\u62ec\uff1a<strong>\u6279\u6b21\u7a33\u5b9a\u6027\u9ad8<\/strong>\uff0c\u6bcf\u5757\u80f6\u7684\u805a\u5408\u6761\u4ef6\u4e00\u81f4\uff0c\u6761\u5e26\u5206\u79bb\u6548\u679c\u53ef\u91cd\u590d;<strong>\u5373\u5f00\u5373\u7528<\/strong>\uff0c\u65e0\u9700\u914d\u5236\u3001\u704c\u80f6\u3001\u7b49\u5f85\u805a\u5408\uff0c\u8282\u770130\u5206\u949f\u4ee5\u4e0a\u65f6\u95f4;<strong>\u517c\u5bb9\u6027\u5f3a<\/strong>\uff0c\u9002\u914d\u4e3b\u6d41\u7535\u6cf3\u69fd\uff0c\u5982Bio-Rad Mini-PROTEAN\u3001Life Technologies XCell\u7b49;<strong>\u5c0f\u5206\u5b50\u86cb\u767d\u5206\u79bb\u6548\u679c\u7a81\u51fa<\/strong>\uff0cBis-Tris\u9884\u5236\u80f6\u5728\u4e2d\u6027pH\u6761\u4ef6\u4e0b\u7535\u6cf3\uff0c\u53ef\u51cf\u5c11\u86cb\u767d\u4fee\u9970\uff0c\u6761\u5e26\u66f4\u9510\u5229\u3002\u9002\u5408<strong>\u9ad8\u901a\u91cf\u5b9e\u9a8c<\/strong>\u3001<strong>\u65f6\u95f4\u7d27\u8feb\u7684\u5b9e\u9a8c<\/strong>\u4ee5\u53ca<strong>\u9700\u8981\u9ad8\u91cd\u590d\u6027\u7684\u5b9e\u9a8c<\/strong>\u3002<\/p>\n<p><strong>\u3000\u3000Q5\uff1a\u91c7\u8d2d\u7535\u6cf3\u8bd5\u5242\u65f6\uff0c\u4f9b\u5e94\u5546\u7684\u672c\u5730\u5316\u670d\u52a1\u4e3a\u4ec0\u4e48\u91cd\u8981?<\/strong><br \/>\nA\uff1a\u672c\u5730\u5316\u670d\u52a1\u53ef\u5e26\u6765\u4ee5\u4e0b\u4ef7\u503c\uff1a<strong>\u73b0\u8d27\u4fdd\u969c<\/strong>\uff0c\u907f\u514d\u5916\u5730\u91c7\u8d2d\u7269\u6d41\u5ef6\u8bef\u3001\u65ad\u8d27\u5bfc\u81f4\u5b9e\u9a8c\u4e2d\u65ad;<strong>\u5feb\u901f\u914d\u9001<\/strong>\uff0c\u7d27\u6025\u9700\u6c42\u53ef\u5f53\u5929\u6216\u6b21\u65e5\u9001\u8fbe;<strong>\u6280\u672f\u54cd\u5e94<\/strong>\uff0c\u5b9e\u9a8c\u9047\u5230\u95ee\u9898\u65f6\uff0c\u672c\u5730\u6280\u672f\u4eba\u5458\u53ef\u73b0\u573a\u6216\u8fdc\u7a0b\u6307\u5bfc\uff0c\u5feb\u901f\u6392\u67e5\u6545\u969c;<strong>\u552e\u540e\u4fbf\u6377<\/strong>\uff0c\u9000\u6362\u8d27\u3001\u4ea7\u54c1\u54a8\u8be2\u6c9f\u901a\u6548\u7387\u9ad8\u3002\u5bf9\u4e8e\u897f\u5357\u5730\u533a\u5b9e\u9a8c\u5ba4\uff0c\u9009\u62e9\u5177\u5907<strong>\u672c\u5730\u4ed3\u50a8\u4e0e\u6280\u672f\u56e2\u961f<\/strong>\u7684\u4f9b\u5e94\u5546\uff0c\u80fd\u663e\u8457\u964d\u4f4e\u91c7\u8d2d\u98ce\u9669\uff0c\u4fdd\u969c\u5b9e\u9a8c\u8fdb\u5ea6\u3002<\/p>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\">\u4f01\u4e1a\u7efc\u5408\u627f\u63a5\u5b9e\u529b\u5c55\u793a<\/h2>\n<p><strong>\u3000\u3000\u6606\u660e\u6625\u65ed\u79d1\u6280\u6709\u9650\u516c\u53f8<\/strong>\u6df1\u8015\u897f\u5357\u79d1\u7814\u670d\u52a1\u9886\u57df\uff0c\u4ee5<strong>\u672c\u5730\u5316\u4ed3\u50a8\u3001\u4e13\u4e1a\u6280\u672f\u652f\u6301\u3001\u5168\u6d41\u7a0b\u4ea7\u54c1\u7ebf<\/strong>\u4e3a\u6838\u5fc3\u4f18\u52bf\uff0c\u4e3a\u4e91\u5357\u53ca\u5468\u8fb9\u533a\u57df\u9ad8\u6821\u5b9e\u9a8c\u5ba4\u3001\u533b\u7597\u673a\u6784\u3001\u751f\u7269\u533b\u836f\u4f01\u4e1a\u3001\u519c\u6797\u79d1\u7814\u5355\u4f4d\u63d0\u4f9b\u4e00\u7ad9\u5f0f\u7535\u6cf3\u8bd5\u5242\u89e3\u51b3\u65b9\u6848\u3002<\/p>\n<p><strong>\u3000\u3000\u4ea7\u54c1\u7ebf\u5168\u8986\u76d6\uff0c\u6ee1\u8db3\u7535\u6cf3\u5168\u6d41\u7a0b\u9700\u6c42<\/strong><br 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kDa\u8303\u56f4\uff0c\u6761\u5e26\u6e05\u6670\u6613\u8fa8\u3002<\/li>\n<li><strong>\u7535\u6cf3\u4e0e\u8f6c\u819c\u7f13\u51b2\u6db2<\/strong>\uff1a\u901f\u6eb6\u7f13\u51b2\u9897\u7c92\uff0c\u52a0\u6c34\u5373\u7528;\u4f4e\u70ed\u91cf\u5feb\u901f\u8f6c\u819c\u6db2\uff0c\u65e0\u9700\u51b0\u6d74\uff0c\u53ef\u7528\u65e0\u6c34\u4e59\u9187\u66ff\u4ee3\u7532\u9187\uff0c\u73af\u4fdd\u4f4e\u6bd2\u3002<\/li>\n<li><strong>\u663e\u8272\u68c0\u6d4b<\/strong>\uff1a\u5206\u68af\u5ea6ECL\u53d1\u5149\u8bd5\u5242\u76d2\uff0c\u6781\u654f\u578b\u53ef\u8fbe\u98de\u514b\u7ea7\u7075\u654f\u5ea6\uff0c37\u5ea6\u5b58\u653e30\u5929\u6027\u80fd\u7a33\u5b9a\u3002<\/li>\n<li><strong>\u86cb\u767d\u5b9a\u91cf<\/strong>\uff1aBCA\u86cb\u767d\u5b9a\u91cf\u8bd5\u5242\u76d2\uff0c\u6807\u51c6\u66f2\u7ebfR2&gt;0.99\uff0c\u5b9a\u91cf\u51c6\u786e\u3002<\/li>\n<\/ul>\n<p><strong>\u3000\u3000\u672c\u5730\u5316\u73b0\u8d27\u4ed3\u50a8\uff0c\u4fdd\u969c\u6781\u901f\u4f9b\u8d27<\/strong><br \/>\n\u516c\u53f8\u642d\u5efa<strong>\u6606\u660e\u672c\u5730\u73b0\u8d27\u4ed3\u50a8\u4f53\u7cfb<\/strong>\uff0c\u5e38\u5907\u5168\u54c1\u7c7b\u6838\u5fc3\u8bd5\u5242\u73b0\u8d27\uff0c\u5305\u62ec\u51dd\u80f6\u8bd5\u5242\u76d2\u3001\u9884\u5236\u80f6\u3001\u8f6c\u819c\u6db2\u3001\u53d1\u5149\u6db2\u7b49\u5e38\u7528\u4ea7\u54c1\u3002\u7701\u5185\u5ba2\u6237\u4e0b\u5355\u540e<strong>\u5feb\u901f\u914d\u9001<\/strong>\uff0c\u7d27\u6025\u9700\u6c42\u53ef\u5f53\u65e5\u9001\u8fbe\uff0c\u5f7b\u5e95\u544a\u522b\u5916\u5730\u91c7\u8d2d\u7269\u6d41\u6162\u3001\u65ad\u8d27\u7f3a\u8d27\u7684\u56f0\u6270\u3002\u9488\u5bf9\u957f\u671f\u5408\u4f5c\u5ba2\u6237\uff0c\u53ef\u63d0\u4f9b<strong>\u5b9a\u671f\u6279\u91cf\u5907\u8d27<\/strong>\u670d\u52a1\uff0c\u786e\u4fdd\u5b9e\u9a8c\u8fdb\u5ea6\u4e0d\u53d7\u5f71\u54cd\u3002<\/p>\n<p><strong>\u3000\u3000\u4e13\u4e1a\u6280\u672f\u56e2\u961f\uff0c\u63d0\u4f9b\u4e00\u5bf9\u4e00\u670d\u52a1<\/strong><br \/>\n\u516c\u53f8\u7ec4\u5efa<strong>\u79d1\u7814\u6280\u672f\u670d\u52a1\u56e2\u961f<\/strong>\uff0c\u5168\u5458\u5177\u5907\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u80cc\u666f\uff0c\u53ef\u63d0\u4f9b\u4ee5\u4e0b\u652f\u6301\uff1a<\/p>\n<ul>\n<li><strong>\u4ea7\u54c1\u9009\u578b\u6307\u5bfc<\/strong>\uff1a\u6839\u636e\u5b9e\u9a8c\u9700\u6c42(\u5982\u86cb\u767d\u5927\u5c0f\u3001\u4e30\u5ea6\u3001\u6837\u672c\u7c7b\u578b)\uff0c\u63a8\u8350\u6700\u5408\u9002\u7684\u51dd\u80f6\u3001\u7f13\u51b2\u6db2\u3001\u53d1\u5149\u6db2\u7ec4\u5408\u3002<\/li>\n<li><strong>\u5b9e\u9a8c\u65b9\u6848\u4f18\u5316<\/strong>\uff1a\u9488\u5bf9\u7591\u96be\u6837\u672c(\u5982\u591a\u7cd6\u591a\u915a\u690d\u7269\u3001\u4f4e\u4e30\u5ea6\u86cb\u767d\u3001\u78f7\u9178\u5316\u86cb\u767d)\uff0c\u63d0\u4f9b\u5b9a\u5236\u5316\u5b9e\u9a8c\u65b9\u6848\u3002<\/li>\n<li><strong>\u95ee\u9898\u6392\u67e5\u4e0e\u552e\u540e<\/strong>\uff1a\u5b9e\u9a8c\u4e2d\u51fa\u73b0\u6761\u5e26\u6a21\u7cca\u3001\u4fe1\u53f7\u5f31\u3001\u8f6c\u819c\u6548\u7387\u4f4e\u7b49\u95ee\u9898\uff0c\u6280\u672f\u4eba\u5458\u53ef\u8fdc\u7a0b\u6216\u73b0\u573a\u6307\u5bfc\uff0c\u5feb\u901f\u5b9a\u4f4d\u5e76\u89e3\u51b3\u3002<\/li>\n<li><strong>\u514d\u8d39\u6280\u672f\u57f9\u8bad<\/strong>\uff1a\u5b9a\u671f\u4e3a\u5408\u4f5c\u5b9e\u9a8c\u5ba4\u63d0\u4f9b\u7535\u6cf3\u5b9e\u9a8c\u64cd\u4f5c\u57f9\u8bad\uff0c\u5e2e\u52a9\u65b0\u624b\u5feb\u901f\u638c\u63e1\u6280\u5de7\u3002<\/li>\n<\/ul>\n<p><strong>\u3000\u3000\u6b63\u54c1\u4fdd\u969c\u4e0e\u54c1\u8d28\u627f\u8bfa<\/strong><br 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\/>\n\u516c\u53f8\u5df2\u670d\u52a1\u7701\u5185\u591a\u6240\u9ad8\u6821\u751f\u547d\u79d1\u5b66\u5b9e\u9a8c\u5ba4\u3001\u533b\u9662\u4e34\u5e8a\u79d1\u7814\u5e73\u53f0\u3001\u751f\u7269\u533b\u836f\u7814\u53d1\u4f01\u4e1a\u3001\u519c\u6797\u68c0\u6d4b\u673a\u6784\uff0c\u51ed\u501f<strong>\u7a33\u5b9a\u7684\u4ea7\u54c1\u54c1\u8d28\u3001\u8d34\u5fc3\u7684\u672c\u5730\u5316\u670d\u52a1\u3001\u9ad8\u6027\u4ef7\u6bd4\u7684\u89e3\u51b3\u65b9\u6848<\/strong>\uff0c\u83b7\u5f97\u4f17\u591a\u5ba2\u6237\u957f\u671f\u5b9a\u70b9\u5408\u4f5c\u4e0e\u6301\u7eed\u590d\u8d2d\u3002\u5ba2\u6237\u53cd\u9988\uff1a<strong>\u8bd5\u5242\u6027\u80fd\u7a33\u5b9a\uff0c\u6761\u5e26\u6e05\u6670\uff0c\u91cd\u590d\u6027\u597d;\u672c\u5730\u670d\u52a1\u54cd\u5e94\u5feb\uff0c\u5b9e\u9a8c\u95ee\u9898\u80fd\u53ca\u65f6\u89e3\u51b3<\/strong>\u3002<\/p>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\">\u9488\u5bf9\u6027\u843d\u5730\u89e3\u51b3\u65b9\u6848<\/h2>\n<p>\u3000\u3000\u9488\u5bf9\u897f\u5357\u5730\u533a\u79d1\u7814\u5b9e\u9a8c\u5ba4\u5728\u7535\u6cf3\u5b9e\u9a8c\u4e2d\u5e38\u89c1\u7684\u75db\u70b9\uff0c\u6606\u660e\u6625\u65ed\u79d1\u6280\u6709\u9650\u516c\u53f8\u63d0\u4f9b\u4ee5\u4e0b<strong>\u9488\u5bf9\u6027\u89e3\u51b3\u65b9\u6848<\/strong>\uff1a<\/p>\n<p><strong>\u3000\u3000\u65b9\u6848\u4e00\uff1a\u89e3\u51b3\u51dd\u80f6\u5236\u5907\u7e41\u7410\u3001\u5f02\u5473\u5927\u3001\u6279\u6b21\u4e0d\u7a33\u95ee\u9898<\/strong><\/p>\n<ul>\n<li><strong>\u63a8\u8350\u4ea7\u54c1<\/strong>\uff1a\u4e00\u6b65\u6cd5\u5feb\u901fPAGE\u51dd\u80f6\u8bd5\u5242\u76d2\u3001\u514d\u67d3\u4e00\u6b65\u6cd5\u51dd\u80f6\u8bd5\u5242\u76d2<\/li>\n<li><strong>\u64cd\u4f5c\u6d41\u7a0b<\/strong>\uff1a\u65e0\u9700\u6dfb\u52a0TEMED\uff0c\u65e0\u523a\u9f3b\u5f02\u5473;\u5c06A\u6db2\u4e0eB\u6db2\u6df7\u5408\u540e\u704c\u80f6\uff0c25\u5206\u949f\u5373\u53ef\u805a\u5408\u5b8c\u6210;\u5f69\u8272\u4e0a\u5c42\u80f6\u65b9\u4fbf\u70b9\u6837\uff0c\u65e0\u9700\u5206\u5c42\u7b49\u5f85\u3002<\/li>\n<li><strong>\u6548\u679c\u4fdd\u969c<\/strong>\uff1a\u51dd\u80f6\u805a\u5408\u5747\u5300\uff0c\u6279\u6b21\u7a33\u5b9a\uff0c\u6761\u5e26\u6e05\u6670\u9510\u5229\uff0c\u5c0f\u5206\u5b50\u86cb\u767d\u5206\u79bb\u6548\u679c\u4f18\u5f02\u3002\u9002\u5408\u5b9e\u9a8c\u5ba4\u65e5\u5e38Western 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