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8px\">\u7b2c\u4e00\u5751\uff1a\u5ffd\u7565\u8bd5\u5242\u517c\u5bb9\u6027\uff0c\u5bfc\u81f4\u5b9e\u9a8c\u4f53\u7cfb\u6df7\u4e71<\/h3>\n<p>\u3000\u3000\u5f88\u591a\u5b9e\u9a8c\u5ba4\u4e3a\u4e86\u964d\u4f4e\u6210\u672c\uff0c\u4f1a\u9009\u62e9\u4e0d\u540c\u54c1\u724c\u7684\u62c6\u5206\u8bd5\u5242\u7ec4\u5408\u4f7f\u7528\uff0c\u6bd4\u5982\u7528A\u54c1\u724c\u7684\u51dd\u80f6\u642d\u914dB\u54c1\u724c\u7684\u8f6c\u819c\u6db2\uff0c\u6216\u7528\u4e0d\u540c\u5382\u5546\u7684\u6297\u4f53\u5b75\u80b2\u8bd5\u5242\uff0c\u6700\u7ec8\u51fa\u73b0\u6761\u5e26\u6a21\u7cca\u3001\u975e\u7279\u5f02\u6027\u4fe1\u53f7\u8fc7\u591a\u3001\u8f6c\u819c\u6548\u7387\u4f4e\u4e0b\u7b49\u95ee\u9898\u3002\u4e0d\u540c\u54c1\u724c\u7684\u8bd5\u5242\u5728pH\u503c\u3001\u7f13\u51b2\u6db2\u6210\u5206\u3001\u6dfb\u52a0\u5242\u6bd4\u4f8b\u4e0a\u5b58\u5728\u5dee\u5f02\uff0c\u8de8\u54c1\u724c\u642d\u914d\u6781\u6613\u5bfc\u81f4\u5b9e\u9a8c\u4f53\u7cfb\u5931\u8861\uff0c\u5c24\u5176\u662f\u7535\u6cf3\u3001\u8f6c\u819c\u3001\u663e\u5f71\u7b49\u73af\u8282\u7684\u8bd5\u5242\uff0c\u5fc5\u987b\u5339\u914d\u540c\u4e00\u54c1\u724c\u7684\u5b8c\u6574\u4f53\u7cfb\u624d\u80fd\u4fdd\u8bc1\u6548\u679c\u3002<\/p>\n<ul>\n<li>\u6b63\u786e\u907f\u5751\u65b9\u5f0f\uff1a\u4f18\u5148\u9009\u62e9\u80fd\u591f\u63d0\u4f9b\u5168\u6d41\u7a0b\u8bd5\u5242\u5957\u88c5\u7684\u670d\u52a1\u5546\uff0c\u786e\u4fdd\u6240\u6709\u8bd5\u5242\u7684\u914d\u65b9\u3001\u7f13\u51b2\u4f53\u7cfb\u4e00\u81f4\uff0c\u4ece\u6e90\u5934\u907f\u514d\u9002\u914d\u6027\u95ee\u9898\u3002<\/li>\n<\/ul>\n<h3 style=\"font-size: 18px;font-weight: bold;margin-top: 16px;margin-bottom: 8px\">\u7b2c\u4e8c\u5751\uff1a\u9009\u62e9\u4f4e\u6027\u4ef7\u6bd4\u8bd5\u5242\uff0c\u727a\u7272\u5b9e\u9a8c\u7a33\u5b9a\u6027<\/h3>\n<p>\u3000\u3000\u90e8\u5206\u5b9e\u9a8c\u5ba4\u4f1a\u88ab\u4f4e\u4ef7\u6742\u724c\u8bd5\u5242\u5438\u5f15\uff0c\u4f46\u5b9e\u9645\u4f7f\u7528\u4e2d\u4f1a\u51fa\u73b0\u4ee5\u4e0b\u95ee\u9898\uff1a<\/p>\n<ul>\n<li>\u6838\u9178\u6216\u86cb\u767d\u63d0\u53d6\u8bd5\u5242\u7eaf\u5ea6\u4e0d\u8db3\uff0c\u5bfc\u81f4\u540e\u7eed\u5b9e\u9a8c\u51fa\u73b0\u6291\u5236\u73b0\u8c61;<\/li>\n<li>\u51dd\u80f6\u7535\u6cf3\u5206\u8fa8\u7387\u5dee\uff0c\u5c0f\u5206\u5b50\u86cb\u5e26\u65e0\u6cd5\u6e05\u6670\u5206\u79bb;<\/li>\n<li>\u53d1\u5149\u6db2\u7075\u654f\u5ea6\u4e0d\u8db3\uff0c\u65e0\u6cd5\u68c0\u6d4b\u4f4e\u4e30\u5ea6\u76ee\u6807\u86cb\u767d;<\/li>\n<li>\u6279\u6b21\u5dee\u5f02\u5927\uff0c\u540c\u4e00\u4ea7\u54c1\u4e0d\u540c\u6279\u6b21\u7684\u5b9e\u9a8c\u7ed3\u679c\u91cd\u590d\u6027\u5dee\u3002<br 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8px\">\u7b2c\u4e09\u5751\uff1a\u5ffd\u7565\u672c\u5730\u5316\u670d\u52a1\uff0c\u65e0\u6cd5\u53ca\u65f6\u89e3\u51b3\u5b9e\u9a8c\u96be\u9898<\/h3>\n<p>\u3000\u3000\u897f\u5357\u5730\u533a\u7684\u79d1\u7814\u5b9e\u9a8c\u5ba4\u666e\u904d\u5b58\u5728\u6280\u672f\u8d44\u6e90\u4e0d\u8db3\u7684\u95ee\u9898\uff0c\u65b0\u624b\u5b9e\u9a8c\u4eba\u5458\u9047\u5230\u64cd\u4f5c\u96be\u9898\u65f6\uff0c\u5f88\u96be\u83b7\u5f97\u53ca\u65f6\u7684\u6280\u672f\u652f\u6301\u3002\u5982\u679c\u9009\u62e9\u5916\u5730\u4f9b\u5e94\u5546\uff0c\u5f80\u5f80\u9700\u8981\u7b49\u5f85\u6570\u5929\u624d\u80fd\u5f97\u5230\u56de\u590d\uff0c\u751a\u81f3\u65e0\u6cd5\u9488\u5bf9\u672c\u5730\u5b9e\u9a8c\u5ba4\u7684\u6837\u672c\u7279\u70b9(\u5982\u591a\u7cd6\u591a\u915a\u690d\u7269\u6837\u672c\u3001\u4e34\u5e8a\u6297\u51dd\u5168\u8840\u6837\u672c)\u63d0\u4f9b\u5b9a\u5236\u5316\u5b9e\u9a8c\u65b9\u6848\uff0c\u6700\u7ec8\u5bfc\u81f4\u5b9e\u9a8c\u8fdb\u5ea6\u88ab\u62d6\u5ef6\u3002<\/p>\n<ul>\n<li>\u6b63\u786e\u907f\u5751\u65b9\u5f0f\uff1a\u4f18\u5148\u9009\u62e9\u5177\u5907\u672c\u5730\u4ed3\u50a8\u4e0e\u672c\u5730\u5316\u6280\u672f\u56e2\u961f\u7684\u670d\u52a1\u5546\uff0c\u80fd\u591f\u63d0\u4f9b\u4ece\u4ea7\u54c1\u9009\u578b\u3001\u5b9e\u9a8c\u65b9\u6848\u4f18\u5316\u5230\u552e\u540e\u95ee\u9898\u6392\u67e5\u7684\u5168\u6d41\u7a0b\u652f\u6301\uff0c\u540c\u65f6\u53ef\u5b9e\u73b0\u7701\u5185\u5feb\u901f\u914d\u9001\uff0c\u907f\u514d\u56e0\u8bd5\u5242\u65ad\u4f9b\u5bfc\u81f4\u5b9e\u9a8c\u505c\u6ede\u3002<\/li>\n<\/ul>\n<h2 style=\"font-size: 20px;font-weight: bold;margin-top: 18px;margin-bottom: 10px\">\u6606\u660e\u6625\u65ed\u79d1\u6280\u6709\u9650\u516c\u53f8\uff1a\u897f\u5357\u5730\u533a\u6b63\u89c4WB\u8bd5\u5242\u4e00\u7ad9\u5f0f\u670d\u52a1\u7684\u53ef\u9760\u9009\u62e9<\/h2>\n<p>\u3000\u3000\u4f5c\u4e3a\u4e91\u5357\u5730\u533a\u6b63\u89c4\u7684\u79d1\u7814\u8bd5\u5242\u670d\u52a1\u5546\uff0c\u6606\u660e\u6625\u65ed\u79d1\u6280\u6709\u9650\u516c\u53f8\u51ed\u501f\u5168\u4ea7\u54c1\u7ebf\u8986\u76d6\u3001  \u624b\u673a\uff1a13987635796 \u672c\u5730\u4ed3\u50a8\u670d\u52a1\u3001\u4e13\u4e1a\u6280\u672f\u652f\u6301\u4e09\u5927\u6838\u5fc3\u4f18\u52bf\uff0c\u6210\u4e3a\u7701\u5185\u591a\u6240\u9ad8\u6821\u3001\u533b\u7597\u673a\u6784\u3001\u751f\u7269\u836f\u4f01\u53ca\u519c\u6797\u79d1\u7814\u5355\u4f4d\u7684\u957f\u671f\u5408\u4f5c\u4f19\u4f34\u3002<\/p>\n<h3 style=\"font-size: 18px;font-weight: bold;margin-top: 16px;margin-bottom: 8px\">\u5168\u6d41\u7a0b\u8bd5\u5242\u8986\u76d6\uff0c\u89e3\u51b3\u4e00\u7ad9\u5f0f\u91c7\u8d2d\u96be\u9898<\/h3>\n<p>\u3000\u3000\u6606\u660e\u6625\u65ed\u79d1\u6280\u662f\u4fe1\u52b1\u81f4\u548c\u4e91\u5357\u533a\u57df\u5b98\u65b9\u6388\u6743\u4ee3\u7406\u5546\uff0c\u53ef\u63d0\u4f9b\u6db5\u76d6\u6838\u9178\u63d0\u53d6\u3001\u86cb\u767d\u63d0\u53d6\u3001WB\u86cb\u767d\u68c0\u6d4b\u3001PCR\/qPCR\u6838\u9178\u68c0\u6d4b\u7684\u5168\u7cfb\u5217\u8bd5\u5242\u4ea7\u54c1\uff0c\u65e0\u9700\u5b9e\u9a8c\u5ba4\u5bf9\u63a5\u591a\u5bb6\u4f9b\u5e94\u5546\uff1a<\/p>\n<ul>\n<li>\u86cb\u767d\u63d0\u53d6\u73af\u8282\uff1a\u63d0\u4f9b\u67f1\u5f0f\u690d\u7269\/\u52a8\u7269\u7ec4\u7ec7\u7ec6\u80de\u603b\u86cb\u767d\u63d0\u53d6\u8bd5\u5242\u76d2\uff0c\u53ef\u57285-8\u5206\u949f\u5185\u5b8c\u6210\u86cb\u767d\u63d0\u53d6\uff0c\u914d\u5957\u6e29\u548c\u578b\u3001\u6807\u51c6\u578b\u3001\u52a0\u5f3a\u578bRIPA\u88c2\u89e3\u6db2\uff0c\u642d\u914d\u901a\u7528\u86cb\u767d\u9176\u6291\u5236\u5242\u4e0e\u78f7\u9178\u9176\u6291\u5236\u5242\uff0c\u6ee1\u8db3\u5929\u7136\u86cb\u767d\u3001\u53d8\u6027\u86cb\u767d\u3001\u78f7\u9178\u5316\u86cb\u767d\u7684\u4e0d\u540c\u5b9e\u9a8c\u9700\u6c42;<\/li>\n<li>\u7535\u6cf3\u5206\u79bb\u73af\u8282\uff1a\u63d0\u4f9b\u4e00\u6b65\u6cd5\u3001\u514d\u67d3\u4e00\u6b65\u6cd5\u3001\u7c7b\u68af\u5ea6\u3001\u4e24\u6b65\u6cd5\u5feb\u901fPAGE\u51dd\u80f6\u8bd5\u5242\u76d2\uff0c\u6452\u5f03\u523a\u6fc0\u6027TEMED\uff0c\u65e0\u523a\u9f3b\u5f02\u5473\uff0c\u5f69\u8272\u4e0a\u5c42\u80f6\u53ef\u65b9\u4fbf\u89c2\u5bdf\u70b9\u6837\u6548\u679c\uff0c\u6574\u5957\u7535\u6cf3\u53ef\u572825\u5206\u949f\u5185\u5b8c\u6210\uff0c\u540c\u65f6\u63d0\u4f9bBis-Tris\u9884\u5236\u80f6\uff0c\u5177\u590712\/15\u5b54\u591a\u79cd\u89c4\u683c\uff0c\u5373\u5f00\u5373\u7528\u4e14\u6279\u6b21\u7a33\u5b9a;<\/li>\n<li>\u8f6c\u819c\u4e0e\u663e\u5f71\u73af\u8282\uff1a\u914d\u5957\u4e09\u8272\u9884\u67d3\u86cb\u767dMarker\u3001\u514d\u8131\u8272\u5feb\u901f\u8003\u9a6c\u65af\u4eae\u84dd\u67d3\u6db2\u3001\u5168\u5957\u7535\u6cf3\/\u8f6c\u819c\u901f\u6eb6\u7f13\u51b2\u9897\u7c92\u3001\u4f4e\u70ed\u91cf\u5feb\u901f\u8f6c\u819c\u6db2\uff0c\u8f6c\u819c\u65e0\u9700\u51b0\u6d74\uff0c\u53ef\u7528\u65e0\u6c34\u4e59\u9187\u66ff\u4ee3\u7532\u9187\uff0c\u73af\u4fdd\u4f4e\u6bd2\uff0c\u540c\u65f6\u63d0\u4f9b\u68af\u5ea6\u578bECL\u53d1\u5149\u8bd5\u5242\u76d2\uff0c\u8986\u76d6\u4e0d\u540c\u4e30\u5ea6\u86cb\u767d\u7684\u68c0\u6d4b\u9700\u6c42\uff0c\u6781\u654f\u578b\u4ea7\u54c1\u53ef\u5b9e\u73b0\u8d85\u4f4e\u98de\u514b\u7ea7\u7075\u654f\u5ea6\uff0c37\u2103\u5b58\u653e30\u5929\u53d1\u5149\u6027\u80fd\u65e0\u660e\u663e\u8870\u51cf\u3002<br \/>\n\u6b64\u5916\uff0c\u6240\u6709\u8bd5\u5242\u5747\u4e3a\u539f\u5382\u76f4\u4f9b\uff0c\u5168\u7a0b\u53ef\u6eaf\u6e90\u3001\u53ef\u67e5\u6279\u6b21\u4fe1\u606f\uff0c\u4ece\u6e90\u5934\u4fdd\u8bc1\u8bd5\u5242\u54c1\u8d28\u7a33\u5b9a\uff0c\u907f\u514d\u8de8\u54c1\u724c\u9002\u914d\u5e26\u6765\u7684\u5b9e\u9a8c\u8bef\u5dee\u3002<\/li>\n<\/ul>\n<h3 style=\"font-size: 18px;font-weight: bold;margin-top: 16px;margin-bottom: 8px\">\u672c\u5730\u4ed3\u50a8\u4e0e\u5feb\u901f\u914d\u9001\uff0c\u89e3\u51b3\u897f\u5357\u5730\u533a\u4f9b\u8d27\u75db\u70b9<\/h3>\n<p>\u3000\u3000\u9488\u5bf9\u897f\u5357\u5730\u533a\u79d1\u7814\u8bd5\u5242\u7269\u6d41\u5468\u671f\u957f\u3001\u65ad\u8d27\u98ce\u9669\u9ad8\u7684\u95ee\u9898\uff0c\u6606\u660e\u6625\u65ed\u79d1\u6280\u642d\u5efa\u4e86\u6606\u660e\u672c\u5730\u73b0\u8d27\u4ed3\u50a8\u4f53\u7cfb\uff0c\u5e38\u5907\u5168\u54c1\u7c7b\u6838\u5fc3\u8bd5\u5242\u73b0\u8d27\uff0c\u53ef\u5b9e\u73b0\u4e91\u5357\u7701\u5185\u5feb\u901f\u914d\u9001\uff0c\u6709\u6548\u907f\u514d\u56e0\u8bd5\u5242\u5ef6\u8fdf\u4f9b\u5e94\u5bfc\u81f4\u7684\u5b9e\u9a8c\u505c\u6ede\u3002\u533a\u522b\u4e8e\u5916\u5730\u4f9b\u5e94\u5546\uff0c\u672c\u5730\u56e2\u961f\u53ef\u5b9e\u73b0\u5f53\u65e5\u54cd\u5e94\u3001\u6b21\u65e5\u9001\u8fbe\u7684\u914d\u9001\u670d\u52a1\uff0c\u4e3a\u5b9e\u9a8c\u5ba4\u7684\u7d27\u6025\u5b9e\u9a8c\u9700\u6c42\u63d0\u4f9b\u53ef\u9760\u4fdd\u969c\u3002<\/p>\n<h3 style=\"font-size: 18px;font-weight: bold;margin-top: 16px;margin-bottom: 8px\">\u4e13\u4e1a\u6280\u672f\u56e2\u961f\u652f\u6301\uff0c\u8986\u76d6\u5168\u6d41\u7a0b\u5b9e\u9a8c\u670d\u52a1<\/h3>\n<p>\u3000\u3000\u6606\u660e\u6625\u65ed\u79d1\u6280\u7ec4\u5efa\u4e86\u5177\u5907\u5206\u5b50\u751f\u7269\u5b9e\u9a8c\u80cc\u666f\u7684\u4e13\u4e1a\u6280\u672f\u670d\u52a1\u56e2\u961f\uff0c\u53ef\u9488\u5bf9\u5b9e\u9a8c\u5ba4\u7684\u5177\u4f53\u9700\u6c42\u63d0\u4f9b\u5b9a\u5236\u5316\u670d\u52a1\uff1a<\/p>\n<ul>\n<li>\u514d\u8d39\u63d0\u4f9b\u4ea7\u54c1\u9009\u578b\u6307\u5bfc\uff0c\u6839\u636e\u5b9e\u9a8c\u5ba4\u7684\u5b9e\u9a8c\u573a\u666f\u3001\u6837\u672c\u7c7b\u578b\u63a8\u8350\u9002\u914d\u7684\u8bd5\u5242\u5957\u88c5;<\/li>\n<li>\u4f18\u5316\u5b9e\u9a8c\u65b9\u6848\uff0c\u9488\u5bf9\u591a\u7cd6\u591a\u915a\u690d\u7269\u6837\u672c\u3001\u4e34\u5e8a\u6297\u51dd\u5168\u8840\u6837\u672c\u7b49\u96be\u5904\u7406\u6837\u672c\uff0c\u63a8\u8350\u4e13\u7528\u7684\u63d0\u53d6\u8bd5\u5242\u4e0e\u5b9e\u9a8c\u6d41\u7a0b;<\/li>\n<li>\u63d0\u4f9b\u552e\u540e\u95ee\u9898\u6392\u67e5\u652f\u6301\uff0c\u5f53\u5b9e\u9a8c\u5ba4\u51fa\u73b0\u5b9e\u9a8c\u7ed3\u679c\u5f02\u5e38\u65f6\uff0c\u6280\u672f\u56e2\u961f\u53ef\u5feb\u901f\u54cd\u5e94\uff0c\u534f\u52a9\u5b9a\u4f4d\u95ee\u9898\u5e76\u7ed9\u51fa\u89e3\u51b3\u65b9\u6848\u3002<br \/>\n\u5bf9\u6bd4\u4f20\u7edf\u5f02\u5730\u4f9b\u5e94\u5546\uff0c\u672c\u5730\u5316\u6280\u672f\u56e2\u961f\u7684\u54cd\u5e94\u901f\u5ea6\u66f4\u5feb\u3001\u9002\u914d\u6027\u66f4\u5f3a\uff0c\u53ef\u6709\u6548\u5e2e\u52a9\u65b0\u624b\u5b9e\u9a8c\u4eba\u5458\u5feb\u901f\u638c\u63e1\u64cd\u4f5c\u6280\u5de7\uff0c\u51cf\u5c11\u5b9e\u9a8c\u8fd4\u5de5\u7684\u6982\u7387\u3002<\/li>\n<\/ul>\n<h3 style=\"font-size: 18px;font-weight: bold;margin-top: 16px;margin-bottom: 8px\">\u771f\u5b9e\u5408\u4f5c\u6848\u4f8b\u9a8c\u8bc1\uff0c\u8986\u76d6\u591a\u7c7b\u79d1\u7814\u573a\u666f<\/h3>\n<p>\u3000\u3000\u6606\u660e\u6625\u65ed\u79d1\u6280\u7684\u4ea7\u54c1\u4e0e\u670d\u52a1\u5df2\u5728\u7701\u5185\u591a\u4e2a\u79d1\u7814\u573a\u666f\u5f97\u5230\u9a8c\u8bc1\uff1a<br \/>\n\u7701\u5185\u9ad8\u6821\u751f\u547d\u79d1\u5b66\u5b9e\u9a8c\u5ba4\u957f\u671f\u5f00\u5c55\u5206\u5b50\u514b\u9686\u3001\u7ec6\u80de\u8f6c\u67d3\u3001\u86cb\u767dWB\u68c0\u6d4b\u7b49\u8bfe\u9898\uff0c\u6b64\u524d\u53d7\u5e02\u9762\u8bd5\u5242\u6279\u6b21\u4e0d\u7a33\u3001\u8d28\u7c92\u5185\u8d85\u6807\u3001\u5b9e\u9a8c\u6d41\u7a0b\u7e41\u7410\u7b49\u95ee\u9898\u5f71\u54cd\uff0c\u5b9e\u9a8c\u8fd4\u5de5\u7387\u9ad8\u3001\u8bfe\u9898\u8fdb\u5ea6\u53d7\u963b\u3002\u4e0e\u6606\u660e\u6625\u65ed\u79d1\u6280\u5408\u4f5c\u540e\uff0c\u5b9e\u9a8c\u5ba4\u7edf\u4e00\u914d\u5957\u65e0\u5185\u8d28\u7c92\u63d0\u53d6\u3001\u5feb\u901fRNA\u63d0\u53d6\u3001\u4e00\u6b65\u6cd5PAGE\u51dd\u80f6\u3001\u9ad8\u7075\u654fECL\u53d1\u5149\u7b49\u5168\u5957\u8bd5\u5242\uff0c\u5927\u5e45\u538b\u7f29\u5b9e\u9a8c\u8017\u65f6\uff0c\u8d28\u7c92\u5185\u542b\u91cf\u6781\u4f4e\uff0c\u9002\u914d\u7ec6\u80de\u8f6c\u67d3\u7b49\u9ad8\u8981\u6c42\u5b9e\u9a8c\uff0c\u6570\u636e\u91cd\u590d\u6027\u663e\u8457\u63d0\u5347\uff0c\u4f9d\u6258\u672c\u5730\u73b0\u8d27\u4ed3\u50a8\u4e0e\u6280\u672f\u652f\u6301\uff0c\u73b0\u5df2\u8fbe\u6210\u591a\u5e74\u5b9a\u70b9\u957f\u671f\u5408\u4f5c\u3002<br 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