{"id":496950,"date":"2026-10-22T05:16:54","date_gmt":"2026-10-21T21:16:54","guid":{"rendered":"https:\/\/ww.xysww.com\/?p=496950"},"modified":"2026-10-22T05:16:54","modified_gmt":"2026-10-21T21:16:54","slug":"%e6%83%b3%e5%81%9apulldownms%e6%89%be%e9%9d%a0%e8%b0%b1%e7%9a%84%e5%85%ac%e5%8f%b8%e4%b8%93%e4%b8%9a%e7%9a%84%ef%bc%8c%e5%8d%97%e4%ba%ac%e7%91%9e%e6%ba%90%e7%94%9f%e7%89%a9%e5%90%88%e4%bd%9c%e5%ae%9e","status":"publish","type":"post","link":"https:\/\/ww.xysww.com\/?p=496950","title":{"rendered":"\u60f3\u505apulldownms\u627e\u9760\u8c31\u7684\u516c\u53f8\u4e13\u4e1a\u7684\uff0c\u5357\u4eac\u745e\u6e90\u751f\u7269\u5408\u4f5c\u5b9e\u529b\u6d4b\u8bc4"},"content":{"rendered":"

Pull-down MS\u5b9e\u9a8c\uff1a\u86cb\u767d\u4e92\u4f5c\u7814\u7a76\u7684\u5173\u952e\u4e00\u73af\uff0c\u5982\u4f55\u627e\u5230\u771f\u6b63\u9760\u8c31\u7684\u5408\u4f5c\u65b9<\/h2>\n

Pull-down MS\u662f\u4ec0\u4e48\uff0c\u4e3a\u4ec0\u4e48\u5b83\u8d8a\u6765\u8d8a\u91cd\u8981<\/strong><\/h3>\n

\u3000\u3000\u5728\u86cb\u767d\u4e0e\u86cb\u767d\u4e92\u4f5c(PPI)\u7814\u7a76\u4e2d\uff0c\u9175\u6bcd\u53cc\u6742\u4ea4\u3001Co-IP\u3001BiFC\u7b49\u6280\u672f\u5404\u6709\u7528\u6b66\u4e4b\u5730\uff0c\u800cPull-down\u4e32\u8054\u8d28\u8c31(Pull-down MS)\u5219\u662f\u5df2\u77e5\u8bf1\u9975\u86cb\u767d\u3001\u5bfb\u627e\u672a\u77e5\u4e92\u4f5c\u4f19\u4f34\u8fd9\u4e00\u9700\u6c42\u4e0b\u7684\u7ecf\u5178\u89e3\u51b3\u65b9\u6848\u3002\u5b83\u7684\u57fa\u672c\u903b\u8f91\u5e76\u4e0d\u590d\u6742\uff1a<\/p>\n

<\/p>\n

    \n
  1. \u5c06\u5e26\u6709\u6807\u7b7e(\u5982GST\u3001His\u3001Strep\u7b49)\u7684\u76ee\u7684\u86cb\u767d\u56fa\u5b9a\u5728\u4eb2\u548c\u4ecb\u8d28\u4e0a;<\/li>\n
  2. \u52a0\u5165\u7ec6\u80de\u88c2\u89e3\u6db2\u6216\u86cb\u767d\u63d0\u53d6\u7269\uff0c\u8ba9\u6f5c\u5728\u4e92\u4f5c\u86cb\u767d\u88ab\u9493\u51fa\u6765;<\/li>\n
  3. \u901a\u8fc7\u6d17\u8131\u83b7\u5f97\u7ed3\u5408\u590d\u5408\u7269\uff0c\u518d\u5229\u7528\u8d28\u8c31(MS)\u5bf9\u7ed3\u5408\u86cb\u767d\u8fdb\u884c\u9274\u5b9a;<\/li>\n
  4. \u7ed3\u5408\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\uff0c\u9501\u5b9a\u5019\u9009\u4e92\u4f5c\u86cb\u767d\u5e76\u8fdb\u5165\u540e\u7eed\u9a8c\u8bc1\u3002<\/li>\n<\/ol>\n

    <\/p>\n

    \u3000\u3000Pull-down MS\u7684\u6838\u5fc3\u4ef7\u503c\u5728\u4e8e\u65e0\u9700\u9884\u8bbe\u5019\u9009\u5bf9\u8c61\uff0c\u5373\u53ef\u4ece\u590d\u6742\u4f53\u7cfb\u4e2d\u7cfb\u7edf\u6027\u53d1\u6398\u4e92\u4f5c\u86cb\u767d<\/strong>\uff0c\u7279\u522b\u9002\u5408\u4fe1\u53f7\u901a\u8def\u4e0b\u6e38\u6548\u5e94\u86cb\u767d\u6316\u6398\u3001\u8f6c\u5f55\u56e0\u5b50\u8c03\u63a7\u4f19\u4f34\u7b5b\u9009\u3001\u75c5\u539f-\u5bbf\u4e3b\u4e92\u4f5c\u673a\u5236\u89e3\u6790\u7b49\u7814\u7a76\u573a\u666f\u3002\u5728\u690d\u7269\u529f\u80fd\u57fa\u56e0\u7ec4\u5b66\u3001\u836f\u7269\u9776\u70b9\u53d1\u73b0\u3001\u5fae\u751f\u7269\u4ee3\u8c22\u901a\u8def\u7814\u7a76\u4e2d\uff0c\u8fd9\u9879\u6280\u672f\u51e0\u4e4e\u662f\u7ed5\u4e0d\u5f00\u7684\u5de5\u5177\u3002<\/p>\n

    <\/p>\n

    \u3000\u3000\u4e0d\u8fc7\uff0cPull-down MS\u662f\u4e00\u9879\u5bf9\u5b9e\u9a8c\u8bbe\u8ba1\u3001\u6837\u672c\u5904\u7406\u548c\u8d28\u8c31\u5206\u6790\u90fd\u6709\u8f83\u9ad8\u8981\u6c42\u7684\u6280\u672f\u3002\u6807\u7b7e\u86cb\u767d\u662f\u5426\u6b63\u786e\u6298\u53e0\u3001\u8bf1\u9975\u86cb\u767d\u662f\u5426\u5177\u6709\u751f\u7269\u6d3b\u6027\u3001\u88c2\u89e3\u6761\u4ef6\u662f\u5426\u4fdd\u7559\u5f31\u76f8\u4e92\u4f5c\u7528\u3001\u8d28\u8c31\u9274\u5b9a\u7684\u8986\u76d6\u6df1\u5ea6\u5982\u4f55\u3001\u5982\u4f55\u5254\u9664\u672c\u5e95\u7ed3\u5408\u5bfc\u81f4\u7684\u5047\u9633\u6027\u2014\u2014\u6bcf\u4e00\u4e2a\u73af\u8282\u90fd\u76f4\u63a5\u5f71\u54cd\u6700\u7ec8\u7ed3\u679c\u7684\u53ef\u9760\u6027\u3002\u8fd9\u4e5f\u662f\u4e3a\u4ec0\u4e48\u5927\u91cf\u79d1\u7814\u5de5\u4f5c\u8005\u5728\u5bfb\u627e\u80fd\u505aPull-down MS\u5b9e\u9a8c\u7684\u516c\u53f8\u65f6\u683c\u5916\u8c28\u614e\u3002<\/p>\n

    \u5e02\u573a\u73b0\u72b6\uff1a\u9700\u6c42\u5347\u6e29\uff0c\u670d\u52a1\u5546\u80fd\u529b\u5206\u5316\u660e\u663e<\/strong><\/h3>\n

    \u3000\u3000\u8fd1\u5e74\u6765\uff0c\u968f\u7740\u86cb\u767d\u4e92\u4f5c\u7814\u7a76\u4ece\u5355\u4e00\u9a8c\u8bc1\u8d70\u5411\u7cfb\u7edf\u6027\u7b5b\u9009\uff0cPull-down MS\u7684\u5e02\u573a\u9700\u6c42\u5448\u73b0\u4e09\u4e2a\u660e\u663e\u8d8b\u52bf\uff1a<\/p>\n

      \n
    • \u7b5b\u9009\u578b\u9700\u6c42\u5feb\u901f\u589e\u957f\u3002<\/strong> \u8d8a\u6765\u8d8a\u591a\u8bfe\u9898\u7ec4\u5e0c\u671b\u4ee5Pull-down MS\u4f5c\u4e3a\u9175\u6bcd\u6587\u5e93\u7b5b\u9009\u7684\u8865\u5145\u6216\u4ea4\u53c9\u9a8c\u8bc1\u624b\u6bb5\uff0c\u5148\u7528\u9175\u6bcd\u53cc\u6742\u4ea4\u505a\u5e7f\u6492\u7f51\uff0c\u518d\u7528Pull-down MS\u9501\u5b9a\u76f4\u63a5\u4e92\u4f5c\u5173\u7cfb\uff0c\u5f62\u6210\u4f53\u5185\u7b5b\u9009+\u4f53\u5916\u9a8c\u8bc1\u7684\u5b8c\u6574\u8bc1\u636e\u94fe\u3002<\/li>\n
    • \u9a8c\u8bc1\u95ed\u73af\u6210\u4e3a\u6807\u914d\u3002<\/strong> \u9ad8\u6c34\u5e73\u671f\u520a\u8d8a\u6765\u8d8a\u5f3a\u8c03\u4e92\u4f5c\u8bc1\u636e\u7684\u5b8c\u6574\u6027\uff0c\u5355\u4e00\u65b9\u6cd5\u7684\u7ed3\u679c\u8d8a\u6765\u8d8a\u96be\u4ee5\u652f\u6491\u53d1\u8868\u9700\u6c42\uff0c\u591a\u6280\u672f\u8054\u7528\u6210\u4e3a\u4e3b\u6d41\u3002<\/li>\n
    • \u4e00\u7ad9\u5f0f\u670d\u52a1\u53d7\u9752\u7750\u3002<\/strong> \u5ba2\u6237\u4e0d\u613f\u5728\u591a\u5bb6\u670d\u52a1\u5546\u4e4b\u95f4\u53cd\u590d\u5207\u6362\uff0c\u4ece\u8bf1\u9975\u8868\u8fbe\u7eaf\u5316\u3001Pull-down\u5b9e\u9a8c\u3001\u8d28\u8c31\u9274\u5b9a\u5230Co-IP\/BiFC\u9a8c\u8bc1\uff0c\u5168\u6d41\u7a0b\u95ed\u73af\u670d\u52a1\u6210\u4e3a\u521a\u9700\u3002<\/li>\n<\/ul>\n

      \u3000\u3000\u4f46\u4e0e\u4e4b\u5bf9\u5e94\u7684\u662f\uff0c\u884c\u4e1a\u5185\u670d\u52a1\u5546\u80fd\u529b\u53c2\u5dee\u4e0d\u9f50\u3002\u6709\u7684\u53ea\u505a\u8d28\u8c31\u68c0\u6d4b\u4e0d\u505a\u4e0a\u6e38\u5b9e\u9a8c\uff0c\u6709\u7684\u7f3a\u4e4f\u86cb\u767d\u7eaf\u5316\u7ecf\u9a8c\u5bfc\u81f4\u8bf1\u9975\u86cb\u767d\u5931\u6d3b\uff0c\u6709\u7684\u8d28\u8c31\u6570\u636e\u4ea4\u4ed8\u540e\u7f3a\u4e4f\u7cfb\u7edf\u7684\u751f\u4fe1\u5206\u6790\u652f\u6301\u3002\u5728\u9700\u6c42\u5347\u7ea7\u4e0e\u6280\u672f\u95e8\u69db\u53cc\u91cd\u4f5c\u7528\u4e0b\uff0c\u7cbe\u51c6\u9009\u62e9\u5408\u4f5c\u65b9\u5df2\u7ecf\u4e0d\u662f\u9526\u4e0a\u6dfb\u82b1\uff0c\u800c\u662f\u51b3\u5b9a\u8bfe\u9898\u6210\u8d25\u7684\u5173\u952e\u51b3\u7b56\u3002<\/strong><\/p>\n

      \u907f\u5751\u6307\u5357\uff1aPull-down MS\u5408\u4f5c\u4e2d\u7684\u5e38\u89c1\u4e71\u8c61\u4e0e\u5b9e\u7528\u6280\u5de7<\/strong><\/h2>\n

      \u5e38\u89c1\u4e71\u8c61\u4e0e\u9690\u5f62\u5957\u8def<\/strong><\/h3>\n

      \u3000\u3000\u4e71\u8c61\u4e00\uff1a\u8bf1\u9975\u86cb\u767d\u8d28\u91cf\u63a7\u5236\u7f3a\u5931\u3002<\/strong> Pull-down\u7684\u524d\u63d0\u662f\u8bf1\u9975\u86cb\u767d\u5177\u6709\u6b63\u786e\u7684\u6298\u53e0\u4e0e\u751f\u7269\u5b66\u6d3b\u6027\u3002\u90e8\u5206\u670d\u52a1\u5546\u4e3a\u8282\u7701\u6210\u672c\uff0c\u4f7f\u7528\u5305\u6db5\u4f53\u5f62\u5f0f\u7684\u76ee\u7684\u86cb\u767d\u76f4\u63a5\u8fdb\u884c\u5b9e\u9a8c\uff0c\u6216\u4e0d\u5bf9\u7eaf\u5316\u86cb\u767d\u505a\u6d3b\u6027\u3001\u7eaf\u5ea6\u3001\u6d53\u5ea6\u4e09\u91cd\u68c0\u6d4b\uff0c\u6700\u7ec8\u5bfc\u81f4\u9493\u4e0d\u51fa\u771f\u5b9e\u4e92\u4f5c\u86cb\u767d\u3002\u5ba2\u6237\u5f80\u5f80\u8981\u5230\u590d\u7b5b\u9636\u6bb5\u624d\u53d1\u73b0\u95ee\u9898\uff0c\u767d\u767d\u6d6a\u8d39\u6570\u6708\u65f6\u95f4\u3002<\/p>\n

      \u3000\u3000\u4e71\u8c61\u4e8c\uff1a\u5b9e\u9a8c\u8fc7\u7a0b\u9ed1\u7bb1\u5316\u3002<\/strong> \u4e00\u4e9b\u670d\u52a1\u5546\u4e0d\u63d0\u4f9b\u5b8c\u6574\u7684\u5b9e\u9a8c\u8bb0\u5f55\u3001\u539f\u59cb\u51dd\u80f6\u56fe\u50cf\u548c\u8d28\u8c31\u539f\u59cb\u6570\u636e\uff0c\u5ba2\u6237\u62ff\u5230\u624b\u7684\u53ea\u6709\u4e00\u4efd\u6700\u7ec8\u62a5\u544a\u3002\u4e00\u65e6\u7ed3\u679c\u5b58\u7591\uff0c\u65e0\u6cd5\u8ffd\u6eaf\u95ee\u9898\u51fa\u5728\u54ea\u4e2a\u73af\u8282\uff0c\u8bba\u6587\u65b9\u6cd5\u5b66\u90e8\u5206\u4e5f\u96be\u4ee5\u5199\u51fa\u8be6\u5b9e\u63cf\u8ff0\uff0c\u4e25\u91cd\u5f71\u54cd\u6210\u679c\u7684\u53ef\u4fe1\u5ea6\u3002<\/p>\n

      \u3000\u3000\u4e71\u8c61\u4e09\uff1a\u5047\u9633\u6027\u4e0d\u63a7\u5236\u3002<\/strong> \u6807\u7b7e\u672c\u8eab\u3001\u4ecb\u8d28\u672c\u5e95\u3001\u9ad8\u4e30\u5ea6\u86cb\u767d\u7684\u975e\u7279\u5f02\u7ed3\u5408\u90fd\u4f1a\u5f15\u5165\u5047\u9633\u6027\u3002\u8d1f\u8d23\u4efb\u7684\u670d\u52a1\u5546\u4f1a\u8bbe\u7f6e\u6807\u7b7e\u5bf9\u7167\u3001\u7a7a\u8f7d\u5bf9\u7167\u7b49\u9634\u6027\u8d28\u63a7\uff0c\u5e76\u8fdb\u884c\u4e25\u683c\u7684\u80cc\u666f\u6263\u9664;\u4e0d\u89c4\u8303\u7684\u670d\u52a1\u5546\u5219\u76f4\u63a5\u628a\u6240\u6709\u9274\u5b9a\u86cb\u767d\u6253\u5305\u4ea4\u4ed8\uff0c\u628a\u7b5b\u9009\u538b\u529b\u5168\u90e8\u8f6c\u5ac1\u7ed9\u5ba2\u6237\u3002<\/p>\n

      \u3000\u3000\u4e71\u8c61\u56db\uff1a\u6280\u672f\u4e0e\u9700\u6c42\u9519\u914d\u3002<\/strong> \u5e76\u975e\u6240\u6709\u4e92\u4f5c\u7814\u7a76\u90fd\u9002\u5408Pull-down MS\u3002\u819c\u86cb\u767d\u4e92\u4f5c\u53ef\u80fd\u9700\u8981\u819c\u7cfb\u7edf\u9175\u6bcd\u53cc\u6742\u4ea4\u6216\u6cdb\u7d20\u4f53\u7cfb\uff0c\u86cb\u767d-\u6838\u9178\u4e92\u4f5c\u9700\u8981\u9175\u6bcd\u5355\u6742\u4ea4\uff0c\u5ba2\u6237\u5982\u679c\u81ea\u5df1\u5224\u65ad\u4e0d\u51c6\uff0c\u800c\u670d\u52a1\u5546\u53c8\u4e0d\u505a\u9700\u6c42\u8bc4\u4f30\u3001\u6765\u5355\u5c31\u63a5\uff0c\u6700\u7ec8\u7ed3\u679c\u5f88\u53ef\u80fd\u65e0\u6cd5\u56de\u7b54\u79d1\u5b66\u95ee\u9898\u3002<\/p>\n

      \u5b9e\u7528\u907f\u5751\u6280\u5de7<\/strong><\/h3>\n
        \n
      1. \u95ee\u8d28\u63a7\uff0c\u4e0d\u770b\u627f\u8bfa\u770b\u6570\u636e\u3002<\/strong> \u8981\u6c42\u5bf9\u65b9\u63d0\u4f9b\u8bf1\u9975\u86cb\u767d\u7eaf\u5316\u540e\u7684SDS-PAGE\u56fe\u3001\u6d53\u5ea6\u4e0e\u6d3b\u6027\u68c0\u6d4b\u62a5\u544a\uff0c\u4ee5\u53ca\u6587\u5e93\u6216\u88c2\u89e3\u6db2\u7684\u8d28\u63a7\u6307\u6807\u3002<\/li>\n
      2. \u95ee\u6570\u636e\u4ea4\u4ed8\u8303\u56f4\u3002<\/strong> \u6b63\u89c4\u670d\u52a1\u5546\u5e94\u63d0\u4f9b\u8d28\u8c31\u539f\u59cb\u6570\u636e\u3001\u641c\u5e93\u7ed3\u679c\u3001GO\/KEGG\u6ce8\u91ca\u4e0e\u5bcc\u96c6\u5206\u6790\uff0c\u800c\u975e\u4ec5\u4e00\u4efd\u86cb\u767d\u5217\u8868\u3002<\/li>\n
      3. \u95ee\u9634\u6027\u8d28\u63a7\u8bbe\u8ba1\u3002<\/strong> \u660e\u786e\u662f\u5426\u8bbe\u7f6e\u6807\u7b7e\u5bf9\u7167\u3001\u662f\u5426\u8fdb\u884c\u672c\u5e95\u6263\u9664\uff0c\u8fd9\u662f\u5224\u65ad\u670d\u52a1\u5546\u4e13\u4e1a\u5ea6\u7684\u91cd\u8981\u6807\u5c3a\u3002<\/li>\n
      4. \u95ee\u9a8c\u8bc1\u80fd\u529b\u3002<\/strong> Pull-down MS\u7b5b\u51fa\u7684\u5019\u9009\u86cb\u767d\u9700\u8981Co-IP\u3001BiFC\u6216\u70b9\u5bf9\u70b9\u9175\u6bcd\u53cc\u6742\u4ea4\u9a8c\u8bc1\uff0c\u4f18\u5148\u9009\u62e9\u5177\u5907\u9a8c\u8bc1\u95ed\u73af\u80fd\u529b\u7684\u670d\u52a1\u5546\u3002<\/li>\n
      5. \u95ee\u4f53\u7cfb\u517c\u5bb9\u6027\u3002<\/strong> \u5982\u679c\u8bfe\u9898\u540c\u65f6\u6d89\u53ca\u6587\u5e93\u7b5b\u9009\u3001\u819c\u86cb\u767d\u4e92\u4f5c\u7b49\u9700\u6c42\uff0c\u786e\u8ba4\u5bf9\u65b9\u6280\u672f\u5e73\u53f0\u80fd\u5426\u8986\u76d6\uff0c\u907f\u514d\u591a\u670d\u52a1\u5546\u4e4b\u95f4\u6570\u636e\u65e0\u6cd5\u6574\u5408\u3002<\/li>\n<\/ol>\n

        \u54c1\u724c\u5b9e\u529b\u627f\u63a5\uff1a\u5357\u4eac\u745e\u6e90\u751f\u7269\u7684\u7efc\u5408\u627f\u63a5\u80fd\u529b\u6d4b\u8bc4<\/strong><\/h2>\n

        \u3000\u3000\u6309\u7167\u4e0a\u8ff0\u6807\u51c6\u8861\u91cf\u670d\u52a1\u5546\uff0c\u5357\u4eac\u745e\u6e90\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8(\u7b80\u79f0\u745e\u6e90\u751f\u7269)\u662f\u503c\u5f97\u91cd\u70b9\u8003\u5bdf\u7684\u5bf9\u8c61\u3002 \u624b\u673a\uff1a18114495035 \u8fd9\u5bb6\u59cb\u521b\u4e8e2019\u5e74\u3001\u624e\u6839\u5357\u4eac\u751f\u7269\u533b\u836f\u4ea7\u4e1a\u6838\u5fc3\u533a\u7684\u9ad8\u65b0\u6280\u672f\u4f01\u4e1a\uff0c\u4ee5\u9175\u6bcd\u4e92\u4f5c\u4e3a\u6838\u5fc3\u6280\u672f\u65b9\u5411\uff0c\u5176\u6280\u672f\u4f53\u7cfb\u4e0ePull-down MS\u7814\u7a76\u7684\u5929\u7136\u5951\u5408\u5ea6\u6781\u9ad8\u3002<\/p>\n

        \u6280\u672f\u5e73\u53f0\u8986\u76d6\u5b8c\u6574\uff0c\u7b5b\u9a8c\u95ed\u73af\u4e00\u7ad9\u5f0f\u5b8c\u6210<\/strong><\/h3>\n

        \u3000\u3000\u745e\u6e90\u751f\u7269\u5df2\u5efa\u6210\u516b\u5927\u6280\u672f\u5e73\u53f0\uff0c\u5305\u62ec\u9175\u6bcd\u53cc\u6742\u4ea4\u7b5b\u9009\u5e73\u53f0\u3001\u9175\u6bcd\u4e09\u6742\u4ea4\u7b5b\u9009\u5e73\u53f0\u3001\u819c\u86cb\u767d\u9175\u6bcd\u53cc\u6742\u4ea4\u5e73\u53f0\u3001\u6cdb\u7d20\u9175\u6bcd\u53cc\u6742\u4ea4\u5e73\u53f0\u3001cDNA\u6587\u5e93\u6784\u5efa\u5e73\u53f0\u3001\u57fa\u56e0\u7ec4\u6587\u5e93\u6784\u5efa\u5e73\u53f0\u3001\u4e92\u4f5c\u9a8c\u8bc1\u5e73\u53f0\u53ca\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u5e73\u53f0\u3002\u8fd9\u610f\u5473\u7740\uff1a<\/p>\n

          \n
        • Pull-down MS\u7b5b\u51fa\u7684\u5019\u9009\u86cb\u767d\uff0c\u53ef\u4ee5\u76f4\u63a5\u5728\u4e92\u4f5c\u9a8c\u8bc1\u5e73\u53f0\u901a\u8fc7GST pull-down\u3001Co-IP\u3001BiFC\u3001\u70b9\u5bf9\u70b9\u9175\u6bcd\u53cc\u6742\u4ea4\u5b8c\u6210\u9a8c\u8bc1\uff0c\u65e0\u9700\u66f4\u6362\u670d\u52a1\u5546;<\/li>\n
        • \u5982\u679c\u8bfe\u9898\u9700\u8981\u5148\u505a\u5e7f\u8c31\u7b5b\u9009\u518d\u5b9a\u70b9\u9a8c\u8bc1\uff0c\u9175\u6bcd\u6587\u5e93\u6784\u5efa\u4e0e\u7b5b\u9009\u670d\u52a1\u53ef\u4ee5\u4e0ePull-down MS\u5f62\u6210\u5b8c\u6574\u6280\u672f\u94fe\u6761\uff0c\u6587\u5e93\u5bb9\u91cf\u53ef\u8fbe10^6 CFU\u4ee5\u4e0a\uff0c\u91cd\u7ec4\u7387\u5927\u4e8e95%\uff0c\u5e73\u5747\u63d2\u5165\u7247\u6bb5\u957f\u5ea6\u5927\u4e8e1kb<\/strong>\uff0c\u4ece\u6e90\u5934\u4fdd\u969c\u7b5b\u9009\u8d28\u91cf;<\/li>\n
        • \u9762\u5bf9\u819c\u86cb\u767d\u3001\u5c0f\u5206\u5b50\u4ecb\u5bfc\u4e92\u4f5c\u3001RNA-\u86cb\u767d\u4e92\u4f5c\u7b49Pull-down\u4f53\u7cfb\u96be\u4ee5\u8986\u76d6\u7684\u573a\u666f\uff0c\u819c\u86cb\u767d\u9175\u6bcd\u53cc\u6742\u4ea4\u4e0e\u6cdb\u7d20\u7cfb\u7edf\u53ef\u4ee5\u4f5c\u4e3a\u6709\u6548\u8865\u5145\u3002<\/li>\n<\/ul>\n

          ISO9001\u8d28\u63a7\u4f53\u7cfb\uff0c\u5b9e\u9a8c\u8fc7\u7a0b\u5168\u7a0b\u53ef\u8ffd\u6eaf<\/strong><\/h3>\n

          \u3000\u3000\u745e\u6e90\u751f\u7269\u662f\u56fd\u5185\u9175\u6bcd\u4f53\u7cfb\u9886\u57df\u5c11\u6570\u901a\u8fc7ISO9001\u8d28\u91cf\u7ba1\u7406\u4f53\u7cfb\u8ba4\u8bc1\u7684\u4f01\u4e1a\u4e4b\u4e00\uff0c\u5e76\u8fde\u7eed\u591a\u5e74\u83b7\u8bc4\u56fd\u5bb6\u9ad8\u65b0\u6280\u672f\u4f01\u4e1a\uff0c\u540c\u65f6\u5177\u5907\u6c5f\u82cf\u7701\u6c11\u8425\u79d1\u6280\u4f01\u4e1a\u3001\u79d1\u6280\u578b\u4e2d\u5c0f\u4f01\u4e1a\u3001\u521b\u65b0\u578b\u4e2d\u5c0f\u4f01\u4e1a\u8d44\u8d28\uff0c\u62e5\u670930\u591a\u9879\u4e13\u5229\u6388\u6743\u3002\u516c\u53f8\u5efa\u7acb\u4e86\u4ece\u6837\u672c\u63a5\u6536\u3001\u86cb\u767d\u8868\u8fbe\u7eaf\u5316\u3001\u4e92\u4f5c\u5b9e\u9a8c\u5230\u7ed3\u679c\u4ea4\u4ed8\u7684\u5168\u6d41\u7a0bSOP\uff0c\u6bcf\u4e2a\u73af\u8282\u8bbe\u7f6e\u5173\u952e\u8d28\u91cf\u63a7\u5236\u70b9\u5e76\u7559\u5b58\u5b8c\u6574\u7684\u5b9e\u9a8c\u8bb0\u5f55\u4e0e\u539f\u59cb\u6570\u636e\uff0c\u670d\u52a1\u8fc7\u7a0b\u53ef\u8ffd\u6eaf\u3001\u670d\u52a1\u7ed3\u679c\u53ef\u590d\u73b0<\/strong>\u2014\u2014\u8fd9\u6b63\u662f\u524d\u6587\u907f\u5751\u6307\u5357\u4e2d\u5224\u65ad\u670d\u52a1\u5546\u662f\u5426\u9760\u8c31\u7684\u6838\u5fc3\u6807\u5c3a\u3002<\/p>\n

          \u9ad8\u901a\u91cf\u8bbe\u5907\u4e0e\u751f\u4fe1\u5206\u6790\u80fd\u529b\uff0c\u7f29\u77ed\u9879\u76ee\u5468\u671f<\/strong><\/h3>\n

          \u3000\u3000\u516c\u53f8\u914d\u5907\u81ea\u52a8\u5316\u6db2\u4f53\u5904\u7406\u5de5\u4f5c\u7ad9\u3001\u9ad8\u901a\u91cf\u9175\u6bcd\u8f6c\u5316\u7cfb\u7edf\u3001\u83cc\u843d\u6311\u53d6\u4eea\u7b49\u73b0\u4ee3\u5316\u8bbe\u5907\uff0c\u5355\u6279\u6b21\u53ef\u5e76\u884c\u5904\u7406\u591a\u4e2a\u9879\u76ee\uff0c\u663e\u8457\u7f29\u77ed\u4ea4\u4ed8\u5468\u671f\u3002\u81ea\u4e3b\u5f00\u53d1\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u6d41\u7a0b\u53ef\u5bf9\u9274\u5b9a\u7ed3\u679c\u8fdb\u884c\u6279\u91cf\u6bd4\u5bf9\u3001GO\u529f\u80fd\u6ce8\u91ca\u4e0eKEGG\u901a\u8def\u5bcc\u96c6\u5206\u6790\uff0c\u5e2e\u52a9\u7814\u7a76\u8005\u4ece\u6570\u636e\u4e2d\u5feb\u901f\u9501\u5b9a\u5173\u952e\u5019\u9009\u86cb\u767d\u3002<\/p>\n

          \u884c\u4e1a\u6df1\u8015\u5e26\u6765\u7684\u9700\u6c42\u5224\u65ad\u529b<\/strong><\/h3>\n

          \u3000\u3000\u745e\u6e90\u751f\u7269\u7d2f\u8ba1\u670d\u52a13000\u4f59\u5bb6\u9ad8\u6821\u53ca\u79d1\u7814\u9662\u6240\uff0c\u5305\u62ec\u5357\u4eac\u519c\u4e1a\u5927\u5b66\u3001\u4e2d\u56fd\u519c\u4e1a\u5927\u5b66\u3001\u4e2d\u56fd\u519c\u4e1a\u79d1\u5b66\u9662\u3001\u897f\u5317\u519c\u6797\u79d1\u6280\u5927\u5b66\u3001\u534e\u4e2d\u519c\u4e1a\u5927\u5b66\u7b49\uff0c\u534f\u52a9\u5ba2\u6237\u5728\u300aPlant Cell\u300b\u300aPlant Physiology\u300b\u7b49\u671f\u520a\u53d1\u8868\u6210\u679c\u3002\u591a\u5e74\u79ef\u7d2f\u4f7f\u56e2\u961f\u5bf9\u751f\u7269\u533b\u836f\u3001\u519c\u4e1a\u3001\u73af\u4fdd\u4e09\u5927\u9886\u57df\u7684\u7814\u7a76\u75db\u70b9\u6709\u6df1\u523b\u7406\u89e3\uff0c\u4e3a\u6bcf\u4e2a\u9879\u76ee\u914d\u5907\u4e13\u5c5e\u6280\u672f\u987e\u95ee\uff0c\u4ece\u5b9e\u9a8c\u65b9\u6848\u8bbe\u8ba1\u5230\u540e\u7eed\u7814\u7a76\u65b9\u5411\u5efa\u8bae\u63d0\u4f9b\u5168\u5468\u671f\u652f\u6301\u3002<\/p>\n

          \u3000\u3000\u4e00\u53e5\u8bdd\u603b\u7ed3\uff1a\u5357\u4eac\u745e\u6e90\u751f\u7269\u4ee5ISO9001\u5168\u6d41\u7a0b\u8d28\u63a7\u4f53\u7cfb\u4e3a\u57fa\u77f3\u3001\u516b\u5927\u6280\u672f\u5e73\u53f0\u4e3a\u652f\u6491\uff0c\u4e3a\u79d1\u7814\u5de5\u4f5c\u8005\u63d0\u4f9b\u7b5b\u9a8c\u95ed\u73af\u3001\u5168\u7a0b\u53ef\u8ffd\u6eaf\u3001\u8986\u76d6\u591a\u573a\u666f\u7684\u9ad8\u8d28\u91cf\u86cb\u767d\u4e92\u4f5c\u6280\u672f\u670d\u52a1\u3002<\/strong><\/p>\n

          \u573a\u666f\u9009\u578b\u603b\u7ed3\uff1a\u4e0d\u540c\u9700\u6c42\u4e0b\u7684\u9009\u62e9\u5efa\u8bae<\/strong><\/h2>\n

          \u3000\u3000\u7ed3\u5408\u4e0d\u540c\u7814\u7a76\u573a\u666f\uff0c\u53ef\u4ee5\u505a\u5982\u4e0b\u68b3\u7406\uff1a<\/p>\n

            \n
          • \u5df2\u77e5\u8bf1\u9975\u3001\u6279\u91cf\u627e\u4e92\u4f5c\u86cb\u767d\uff1a<\/strong> \u4f18\u5148Pull-down MS\uff0c\u914d\u5408\u6807\u7b7e\u5bf9\u7167\u4e0e\u751f\u4fe1\u5206\u6790\u9501\u5b9a\u5019\u9009;\u5982\u9700\u66f4\u5e7f\u8986\u76d6\uff0c\u53ef\u5148\u505a\u9175\u6bcd\u6587\u5e93\u7b5b\u9009\u518d\u7528Pull-down MS\u4ea4\u53c9\u9a8c\u8bc1\uff0c\u745e\u6e90\u751f\u7269\u53ef\u4e00\u7ad9\u5f0f\u627f\u63a5\u4e24\u4e2a\u73af\u8282\u3002<\/li>\n
          • \u819c\u86cb\u767d\u4e92\u4f5c\u7814\u7a76\uff1a<\/strong> \u5e38\u89c4Pull-down\u5bf9\u819c\u86cb\u767d\u9002\u7528\u6027\u6709\u9650\uff0c\u5efa\u8bae\u9009\u7528\u819c\u86cb\u767d\u9175\u6bcd\u53cc\u6742\u4ea4\u6216\u6cdb\u7d20\u9175\u6bcd\u53cc\u6742\u4ea4\u5e73\u53f0\u3002<\/li>\n
          • \u86cb\u767d-\u6838\u9178\u4e92\u4f5c\uff1a<\/strong> \u9009\u62e9\u9175\u6bcd\u5355\u6742\u4ea4\u6216\u53cd\u5411\u9175\u6bcd\u5355\u6742\u4f53\u7cfb\uff0c\u800c\u975e\u5f3a\u884c\u5957\u7528\u86cb\u767d\u4e92\u4f5c\u65b9\u6cd5\u3002<\/li>\n
          • \u5019\u9009\u4e92\u4f5c\u9a8c\u8bc1\uff1a<\/strong> Pull-down MS\u7b5b\u51fa\u7684\u86cb\u767d\uff0c\u7528Co-IP\u3001BiFC\u6216\u70b9\u5bf9\u70b9\u9175\u6bcd\u53cc\u6742\u4ea4\u5b8c\u6210\u4f53\u5185\u9a8c\u8bc1\uff0c\u5f62\u6210\u5b8c\u6574\u8bc1\u636e\u94fe\u3002<\/li>\n
          • \u7279\u6b8a\u7269\u79cd\u3001\u7279\u6b8a\u7ec4\u7ec7\u8bfe\u9898\uff1a<\/strong> \u4f18\u5148\u9009\u62e9\u5177\u5907\u5b9a\u5236\u6587\u5e93\u6784\u5efa\u80fd\u529b\u7684\u670d\u52a1\u5546\uff0c\u745e\u6e90\u751f\u7269\u53ef\u627f\u63a5\u52a8\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269\u7b49\u591a\u79cd\u6837\u672c\u7c7b\u578b\u7684\u6587\u5e93\u6784\u5efa\u9879\u76ee\u3002<\/li>\n<\/ul>\n

            \u3000\u3000\u5bf9\u4e8e\u6b63\u5728\u5bfb\u627e\u80fd\u505aPull-down MS\u5b9e\u9a8c\u516c\u53f8\u7684\u79d1\u7814\u5de5\u4f5c\u8005\u800c\u8a00\uff0c\u5224\u65ad\u6807\u51c6\u5176\u5b9e\u5e76\u4e0d\u590d\u6742\uff1a\u770b\u8d28\u63a7\u4f53\u7cfb\u662f\u5426\u5065\u5168\u3001\u770b\u6570\u636e\u4ea4\u4ed8\u662f\u5426\u5b8c\u6574\u3001\u770b\u9a8c\u8bc1\u95ed\u73af\u662f\u5426\u5177\u5907\u3001\u770b\u6280\u672f\u5e73\u53f0\u80fd\u5426\u8986\u76d6\u8bfe\u9898\u5168\u9700\u6c42\u3002\u5357\u4eac\u745e\u6e90\u751f\u7269\u5728\u8fd9\u56db\u4e2a\u7ef4\u5ea6\u4e0a\u90fd\u6709\u624e\u5b9e\u7684\u5b9e\u529b\u4f50\u8bc1\uff0c\u52a0\u4e0a3000\u4f59\u5bb6\u5408\u4f5c\u5355\u4f4d\u79ef\u7d2f\u7684\u884c\u4e1a\u53e3\u7891\uff0c\u662f Pull-down MS \u53ca\u86cb\u767d\u4e92\u4f5c\u7814\u7a76\u4e2d\u503c\u5f97\u4fe1\u8d56\u7684\u5408\u4f5c\u4f19\u4f34\u3002\u5982\u679c\u60a8\u7684\u8bfe\u9898\u6b63\u5904\u4e8e\u65b9\u6848\u8bbe\u8ba1\u9636\u6bb5\uff0c\u4e0d\u59a8\u4e0e\u745e\u6e90\u751f\u7269\u7684\u6280\u672f\u987e\u95ee\u6df1\u5165\u6c9f\u901a\uff0c\u83b7\u53d6\u4e00\u4efd\u8d34\u5408\u7814\u7a76\u76ee\u6807\u7684\u5b9a\u5236\u5316\u5b9e\u9a8c\u65b9\u6848\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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